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Titolo:
PREVOTELLA-INTERMEDIA FIMBRIAE MEDIATE HEMAGGLUTINATION
Autore:
LEUNG KP; FUKUSHIMA H; NESBITT WE; CLARK WB;
Indirizzi:
UNIV FLORIDA,JHMHC,DEPT ORAL BIOL,PERIODONTAL DIS RES CTR,COLL DENT,BOX 100424 GAINESVILLE FL 32610 OSAKA DENT UNIV,DEPT BACTERIOL OSAKA JAPAN
Titolo Testata:
Oral microbiology and immunology
fascicolo: 1, volume: 11, anno: 1996,
pagine: 42 - 50
SICI:
0902-0055(1996)11:1<42:PFMH>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLACK-PIGMENTED BACTEROIDES; ESCHERICHIA-COLI; EPITHELIAL-CELLS; PERIODONTAL-DISEASES; ADHESIN HA-AG2; GINGIVALIS; ATTACHMENT; ADHERENCE; ANTIBODY; ASACCHAROLYTICUS;
Keywords:
PREVOTELLA INTERMEDIA; FIMBRIA; HEMAGGLUTINATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
K.P. Leung et al., "PREVOTELLA-INTERMEDIA FIMBRIAE MEDIATE HEMAGGLUTINATION", Oral microbiology and immunology, 11(1), 1996, pp. 42-50

Abstract

Our earlier studies demonstrated that clinical strains of Prevotella intermedia, isolated from human periodontal pockets, possess various types of fimbria (surface appendages) as determined ultrastructurally. ? These bacteria have the ability to agglutinate selected mammalian erythrocytes. Hemagglutinating activity exhibited by these cells may be attributable to these surface structures. Strain 17, which posseses fimbriae of 8 nm in diameter and readily agglutinates human, monkey, sheep, rabbit, and mouse erythrocytes was selected to determine whether these fimbriae possessed the hemagglutinating activity. Fimbriae were mechanically sheared, concentrated by ammonium sulfate precipitation, solubilized in 10 mM Tris-HCl buffer containing 0.5% deoxycholate and partly purified by ultracentrifugation in a 10-50% linear sucrose gradient. Isolated fimbriae banded at a density of 1.10-1.15 g/ml, appeared fairly uniform ultrastructurally, and possessed hemagglutinating activity. The hemagglutinating activity of P. intermedia whole cells and isolated fimbriae was reduced by treatment with proteases and eliminatedby treatment with heat at 80 degrees C for 10 min. The optimal pH forthe hemagglutination was 7.0. In the process of hemagglutination, P. intermedia whole cells and isolated fimbriae bound to rabbit erythrocytes as observed by: (a) decrease in the hemagglutinating activity of bacterial whole cells and isolated fimbriae after incubation with rabbit erythrocytes, (b) increase in hemagglutinating activity of the red cells used for absorption and (c) the presence of P. intermedia whole cells and fimbriae on red cells after absorption as revealed by immunoelectron microscopy. Both the immune immunoglobulin G of the rabbit polyclonal antifimbriae antibody and the immune immunoglobulin G and its Fab fragments of the murine monoclonal anti-fimbriae antibody were effective inhibitors of hemagglutination mediated by isolated fimbriae. Immunogold labeling showed that the monoclonal antibody bound specifically to P, intermedia fimbriae. These results collectively suggest thatthe hemagglutination exhibited by P. intermedia may be attributable to the fimbriae or the fimbrial-associated components.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:04:36