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Titolo:
CHROMOSOMAL DISTRIBUTION OF THE HAMSTER INTRACISTERNAL A-PARTICLE (IAP) RETROTRANSPOSONS
Autore:
ALTENSCHILDESCHE GMZ; HELLER H; WILGENBUS P; TJIA ST; DOERFLER W;
Indirizzi:
UNIV COLOGNE,INST GENET,WEYERTAL 121 D-50931 COLOGNE GERMANY UNIV COLOGNE,INST GENET D-50931 COLOGNE GERMANY
Titolo Testata:
Chromosoma
fascicolo: 5, volume: 104, anno: 1996,
pagine: 341 - 344
SICI:
0009-5915(1996)104:5<341:CDOTHI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
TERMINAL REPEAT SEQUENCES; MOUSE GENOME; GENES; DNA; ORGANIZATION; ELEMENTS; EVOLUTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
G.M.Z. Altenschildesche et al., "CHROMOSOMAL DISTRIBUTION OF THE HAMSTER INTRACISTERNAL A-PARTICLE (IAP) RETROTRANSPOSONS", Chromosoma, 104(5), 1996, pp. 341-344

Abstract

The retrotransposon-like elements of the intracisternal A-particle GAP) sequences occur in about 900 copies per haploid hamster cell genome. By applying the fluorescent in situ hybridization (FISH) technique and four different, cloned segments of the IAP element as hybridizationprobes, these elements were found to be distributed in specific patterns over many of the 44 hamster chromosomes. The hybridization patterns were very similar regardless of whether all four probes or only the IAPI probe carrying the long terminal repeat (LTR) region were used. The IAP elements were found most abundantly, though not exclusively, onthe short arms of at least 12 of the autosomes. Of the sex chromosomes, the shorter Y chromosome was stained on both arms, and the X chromosome on one arm by the IAP probes. Primary Syrian hamster cells, the established Syrian hamster cell line BHK21, and the adenovirus type 12 (Ad12)-transformed BHK21 cell line T637 yielded very similar results. In Chinese hamster ovary (CHO) or 3T3 mouse cells, signals could not be elicited by FISH using the Syrian hamster IAP probes. On Southern blots, the DNAs from these cell lines hybridized very weakly, if at all,to the IAP sequences. Thus, IAP sequences were retroposed after Syrian hamster and mouse or Syrian and Chinese hamsters had diverged in evolution.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 16:04:27