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Titolo:
IDENTIFICATION OF DNA GYRASE A MUTATIONS IN CIPROFLOXACIN-RESISTANT ISOLATES OF SALMONELLA-TYPHIMURIUM FROM MEN AND CATTLE IN GERMANY
Autore:
HEISIG P; KRATZ B; HALLE E; GRASER Y; ALTWEGG M; RABSCH W; FABER JP;
Indirizzi:
UNIV BONN,MECKENHEIMER ALLEE 168 D-53115 BONN GERMANY BEREICH MED CHARITE D-10117 BERLIN GERMANY UNIV ZURICH CH-8028 ZURICH SWITZERLAND BUNDESINST GESUNDHEITLICHEN VERBRAUCHERSCHUTZ D-38843 WERNIGERODE GERMANY VET MED BEREICH WERNIGERODE D-38843 WERNIGERODE GERMANY UNIV BONN D-53127 BONN GERMANY
Titolo Testata:
Microbial drug resistance
fascicolo: 3, volume: 1, anno: 1995,
pagine: 211 - 218
SICI:
1076-6294(1995)1:3<211:IODGAM>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; ESCHERICHIA-COLI; QUINOLONE RESISTANCE; STAPHYLOCOCCUS-AUREUS; DETERMINING REGION; PHAGE TYPE-204C; A-PROTEIN; GENE; STRAINS; TOPOISOMERASES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
P. Heisig et al., "IDENTIFICATION OF DNA GYRASE A MUTATIONS IN CIPROFLOXACIN-RESISTANT ISOLATES OF SALMONELLA-TYPHIMURIUM FROM MEN AND CATTLE IN GERMANY", Microbial drug resistance, 1(3), 1995, pp. 211-218

Abstract

Six multiply resistant isolates of Salmonella typhimurium var. copenhagen with high-level resistance to fluoroquinolones (e.g., MIC of ciprofloxacin: 32 mu g/ml) were isolated from human patients (n = 3) and from cattle (n = 3). The isolates were examined by complementation tests using a set of broad-host-range plasmids, which carry either the gyrA(+) or the gyrB(+) genes or a combination of both from Escherichia coli K-12. The results indicated a combination of gyrA and gyrB mutations in all isolates. Subsequent direct sequencing of PCR-generated internal DNA fragments of gyrA revealed an identical double mutation in allsix isolates (Ser-83 --> Ala and Asp-87 --> Asn). In addition, the results of phenotypic (i.e., phagetype, biotype, serotype) and genotypiccharacterization [i.e., ribotyping and polymerase chain reaction fingerprinting (PCR-fingerprinting)] were identical for all six isolates and were distinguishable from a quinolone-susceptible strain of the same serovar and an unrelated isolate of S. typhimurium. These data indicate the clonal identity of the fluoroquinolone-resistant strains of S.typhimurium isolated from men and cattle in Germany.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 03:22:43