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Titolo:
CIRCULATING HUMAN DENDRITIC CELLS DIFFERENTIALLY EXPRESS HIGH-LEVELS OF A 55-KD ACTIN-BUNDLING PROTEIN
Autore:
MOSIALOS G; BIRKENBACH M; AYEHUNIE S; MATSUMURA F; PINKUS GS; KIEFF E; LANGHOFF E;
Indirizzi:
MASSACHUSETTS GEN HOSP,RENAL UNIT BOSTON MA 02114 MASSACHUSETTS GEN HOSP,RENAL UNIT BOSTON MA 02114 HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET & MED BOSTON MA 00000 DANA FARBER CANC INST,DEPT HUMAN RETROVIROL BOSTON MA 02115 BRIGHAM & WOMENS HOSP,DEPT PATHOL BOSTON MA 02115 UNIV CHICAGO,MARJORIE B KOVLER VIRAL ONCOL LABS CHICAGO IL 60637 RUTGERS STATE UNIV,DEPT MOLEC BIOL & BIOCHEM PISCATAWAY NJ 08855
Titolo Testata:
The American journal of pathology
fascicolo: 2, volume: 148, anno: 1996,
pagine: 593 - 600
SICI:
0002-9440(1996)148:2<593:CHDCDE>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
LANGERHANS CELLS; MONOCLONAL-ANTIBODIES; LYMPHOCYTES; TISSUES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
G. Mosialos et al., "CIRCULATING HUMAN DENDRITIC CELLS DIFFERENTIALLY EXPRESS HIGH-LEVELS OF A 55-KD ACTIN-BUNDLING PROTEIN", The American journal of pathology, 148(2), 1996, pp. 593-600

Abstract

This study was initiated to examine the differential expression of anevolutionary conserved human 55-kd actin-bundling (p55) protein that is induced in B lymphocytes by Epstein-Barr virus infection. Our studydemonstrates that p55 is specifically expressed at constitutively high levels in human peripheral blood dendritic cells and lymph node (interdigitating) dendritic cells. Blood dendritic cells constitute a minority (<2%) of all blood leukocytes but are a distinct population of potent antigen-presenting cells. Immunofluorescence microscopy with a monoclonal antibody specific for p55 showed that 87% of peripheral blooddendritic cells stained brightly in the cytoplasm and in the veiled cytoplasmic extensions. In contrast, monocytes, granulocytes, T cells, and B lymphocytes showed no expression of the p55 protein. Western blot analysis confirmed that only the dendritic cell component of peripheral blood expresses high levels of p55. Staining of human lymph node sections demonstrated selective expression of the p55 antigen by dendritic cells in the T-cell-dependent areas but not in the B cell follicles. p55 is likely to be involved in the organization of a specialized microfilament cytoskeleton in the dendritic cells, and the anti-p55 antibody should be useful for further characterization of this important population of antigen-presenting cells in clinical transplantation, HIV-1 pathogenesis, and autoimmune diseases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 05:26:07