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Titolo:
DETECTION OF 11Q13 REARRANGEMENTS IN HEMATOLOGIC NEOPLASIAS BY DOUBLE-COLOR FLUORESCENCE IN-SITU HYBRIDIZATION
Autore:
COIGNET LJA; SCHUURING E; KIBBELAAR RE; RAAP TK; KLEIVERDA KK; BERTHEAS MF; WIEGANT J; BEVERSTOCK G; KLUIN PM;
Indirizzi:
LEIDEN UNIV,DEPT PATHOL,POB 9600 2300 RC LEIDEN NETHERLANDS LEIDEN UNIV,DEPT PATHOL 2300 RC LEIDEN NETHERLANDS LEIDEN UNIV,DEPT CYTOCHEM & CYTOMETRY 2300 RC LEIDEN NETHERLANDS LEIDEN UNIV,DEPT HUMAN GENET 2300 RC LEIDEN NETHERLANDS CHRU,LAB CYTOGENET HEMATOL ST ETIENNE FRANCE
Titolo Testata:
Blood
fascicolo: 4, volume: 87, anno: 1996,
pagine: 1512 - 1519
SICI:
0006-4971(1996)87:4<1512:DO1RIH>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERMEDIATE LYMPHOCYTIC LYMPHOMA; NON-HODGKINS-LYMPHOMA; CHROMOSOME-11 BCL-1 LOCUS; B-CELL LYMPHOMAS; CYCLIN D1 GENE; CENTROCYTIC LYMPHOMA; INSITU HYBRIDIZATION; BREAKPOINT REGION; TRANSLOCATION; ABNORMALITIES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
L.J.A. Coignet et al., "DETECTION OF 11Q13 REARRANGEMENTS IN HEMATOLOGIC NEOPLASIAS BY DOUBLE-COLOR FLUORESCENCE IN-SITU HYBRIDIZATION", Blood, 87(4), 1996, pp. 1512-1519

Abstract

Rearrangements within the chromosome 11q13 region are frequent in hematologic malignancies. 50% to 75% of mantle cell lymphomas (MCLs) carry a translocation t(11;14) (q13;q32). Using Southern blot analysis, a BCL1 breakpoint can be detected in approximately 50% of MCLs. It is not known whether other MCLs harbor also breakpoints at 11q13. Breakpoints in this region not involved in t(11;14), are detected in chronic lymphocytic leukemia and acute myeloid leukemia. To detect and localize breakpoints at 11q13 more accurately, we have developed fluorescence in situ hybridization using two probe sets of differently labeled cosmids, symmetrically localized at either side of the major translocation cluster of BCL1. These probes span a region of 450 to 750 kb. We applied this assay to a series of hematologic malignancies with 11q13 abnormalities identified by classical cytogenetics. All four samples with at(11;14) (q13;q32) showed dissociation of the differently colored signals in metaphase and interphase cells, thereby indicating a chromosomal break in the region defined by the probe sets. The frequency of abnormal metaphase and interphase cells was comparable with that observedby banding analysis. No dissociation was observed in any of the 13 malignancies with other chromosomal 11q13 abnormalities, indicating thatthese chromosomal breaks occurred outside the 450- to 750-kb region covered by the probes. One patient showed triplication and one patient showed monoallelic loss of this region. The current data show that double-color fluorescence in situ hybridization is a simple and reliable method for detection of the t(11;14)(q13;q32) in interphase cell nuclei and that it can be used to distinguish this translocation from other11q13 rearrangements in hematologic malignancies. (C) 1996 by The American Society of Hematology.

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Documento generato il 03/12/20 alle ore 12:07:48