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Titolo:
BASIC FIBROBLAST GROWTH-FACTOR OVEREXPRESSION IN ENDOTHELIAL-CELLS - AN AUTOCRINE MECHANISM FOR ANGIOGENESIS AND ANGIOPROLIFERATIVE DISEASES
Autore:
GUALANDRIS A; RUSNATI M; BELLERI M; NELLI EE; BASTAKI M; MOLINARITOSATTI MP; BONARDI F; PAROLINI S; ALBINI A; MORBIDELLI L; ZICHE M; CORALLINI A; POSSATI L; VACCA A; RIBATTI D; PRESTA M;
Indirizzi:
UNIV BRESCIA,DEPT BIOMED SCI & BIOTECHNOL,UNIT HISTOL,VIA VALSABBINA 19 I-25123 BRESCIA ITALY UNIV BRESCIA,DEPT BIOMED SCI & BIOTECHNOL,UNIT HISTOL I-25123 BRESCIAITALY IST NAZL RIC CANC I-16132 GENOA ITALY UNIV BRESCIA,DEPT BIOMED SCI & BIOTECHNOL,UNIT GEN PATHOL & IMMUNOL I-25123 BRESCIA ITALY UNIV FLORENCE,DEPT PHARMACOL I-50134 FLORENCE ITALY UNIV FERRARA,INST MICROBIOL I-44100 FERRARA ITALY UNIV FERRARA,INTERDEPT CTR BIOTECHNOL I-44100 FERRARA ITALY UNIV ANCONA,INST BIOMED SCI I-60100 ANCONA ITALY UNIV BARI,DEPT BIOMED SCI & HUMAN ONCOL I-70124 BARI ITALY UNIV BARI,INST ANAT HISTOL & EMBRYOL I-70124 BARI ITALY
Titolo Testata:
Cell growth & differentiation
fascicolo: 2, volume: 7, anno: 1996,
pagine: 147 - 160
SICI:
1044-9523(1996)7:2<147:BFGOIE>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR GENE-EXPRESSION; RECONSTITUTED BASEMENT-MEMBRANE; CAPILLARY-LIKE STRUCTURES; NIH 3T3 CELLS; EXTRACELLULAR-MATRIX; FACTOR BFGF; KAPOSIS SARCOMA; TUMOR-GROWTH; UROKINASE RECEPTOR; INTERFERON-ALPHA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
89
Recensione:
Indirizzi per estratti:
Citazione:
A. Gualandris et al., "BASIC FIBROBLAST GROWTH-FACTOR OVEREXPRESSION IN ENDOTHELIAL-CELLS - AN AUTOCRINE MECHANISM FOR ANGIOGENESIS AND ANGIOPROLIFERATIVE DISEASES", Cell growth & differentiation, 7(2), 1996, pp. 147-160

Abstract

Basic fibroblast growth factor (bFGF) is expressed in vascular endothelium during tumor neovascularization and angioproliferative diseases,The ultimate significance of this observation is poorly understood, We have investigated the biological consequences of endothelial cell activation by endogenous bFGF in a mouse aortic endothelial cell line stably transfected with a retroviral expression vector harboring a humanbFGF cDNA, Selected clones expressing M(r) 24,000, M(r) 22,000, and/or M(r) 18,000 bFGF isoforms were characterized by a transformed morphology and an increased saturation density, bFGF transfectants showed invasive behavior and sprouting activity in three-dimensional fibrin gels and formed a complex network of branching cord-like structures connecting foci of infiltrating cells when seeded on laminin-rich basement membrane matrix (Matrigel). The invasive and morphogenetic behavior was prevented by anti-bFGF antibody, revealing the autocrine modality ofthe process, The biological consequences of this autocrine activationwere investigated in vivo, bFGF-transfected cells gave rise to highlyvascularized lesions resembling Kaposi's sarcoma when injected in nude mice and induced angiogenesis in avascular rabbit cornea, When injected into the allantoic sac of the chick embryo, they caused an increase in vascular density and formation of hemangiomas in the chorioallantoic membrane, In conclusion, bFGF-overexpressing endothelial cells acquire an angiogenic phenotype and recruit quiescent endothelium originating angioproliferative lesions in vivo, These findings demonstrate that bFGF overexpression exerts an autocrine role for endothelial cells and support the notion that tumor neovascularization and angioproliferative diseases can be triggered by stimuli that induce vascular endothelium to produce its own autocrine factor(s).

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Documento generato il 19/09/20 alle ore 12:29:57