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Titolo:
A MEMBER OF THE PHYLOGENETICALLY CONSERVED CAD FAMILY OF TRANSCRIPTIONAL REGULATORS IS DRAMATICALLY UP-REGULATED DURING THE PROGRAMMED CELL-DEATH OF SKELETAL-MUSCLE IN THE TOBACCO HAWKMOTH MANDUCA-SEXTA
Autore:
SUN DH; SATHYANARAYANA UG; JOHNSTON SA; SCHWARTZ LM;
Indirizzi:
UNIV MASSACHUSETTS,MOLEC & CELLULAR BIOL PROGRAM AMHERST MA 01003 UNIV MASSACHUSETTS,DEPT BIOL AMHERST MA 01003 UNIV TEXAS,SW MED CTR,DEPT MED & BIOCHEM DALLAS TX 75235
Titolo Testata:
Developmental biology
fascicolo: 2, volume: 173, anno: 1996,
pagine: 499 - 509
SICI:
0012-1606(1996)173:2<499:AMOTPC>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
TAT-BINDING-PROTEIN; SACCHAROMYCES-CEREVISIAE; NEURONAL DEATH; 26-S PROTEASE; RNA-SYNTHESIS; C-MYC; ACTIVATION; APOPTOSIS; GENE; INHIBITORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
D.H. Sun et al., "A MEMBER OF THE PHYLOGENETICALLY CONSERVED CAD FAMILY OF TRANSCRIPTIONAL REGULATORS IS DRAMATICALLY UP-REGULATED DURING THE PROGRAMMED CELL-DEATH OF SKELETAL-MUSCLE IN THE TOBACCO HAWKMOTH MANDUCA-SEXTA", Developmental biology, 173(2), 1996, pp. 499-509

Abstract

The intersegmental muscles (ISMs) of the tobacco hawkmoth Manduca sexta participate in the emergence behavior of the adult moth at the end of metamorphosis and then die during the subsequent 30-hr period. The trigger for this death is a decline in the circulating titer of the insect molting hormone 20-hydroxyecdysone (20-HE). Previous work has demonstrated that the ability of the ISMs to die is dependent on new geneexpression. Using a differential hybridization cloning strategy, a cDNA library made from the ISMs committed to die was screened, and four up-regulated clones were isolated. One clone, 18-56, was selected for this study. Northern and Western analysis demonstrated that while clone 18-56 was expressed in all tissues examined and during every stage of ISM development, there was a dramatic increase in expression at bothmRNA and protein levels when the ISMs became committed to die. If ISMdeath was delayed by an injection of 20-HE on the day proceeding adult emergence, 18-56 expression remained at basal levels. Immunocytochemistry demonstrated that 18-56 protein was located predominantly in nuclei prior to the commitment of the ISMs to die and then accumulated tohigh levels in cytoplasm at the time of cell death. DNA sequence analysis revealed that 18-56 protein shares 74% identity with yeast SUG1 and 92% with human Trip1, both of which are members of the conserved CAD (Conserved ATPase-containing Domain) family of putative transcriptional regulators. To verify that these genes shared functional as well as sequence homology, Manduca clone 18-56 was transformed into a yeast mutant for SUG1 function. Manduca 18-56 was able to both complement the lethal SUG1 phenotype and to suppress the transcriptional activity of a SUG1 mutation in yeast. Taken together, these data support the hypothesis that members of the phylogenetically conserved CAD family participate in important basal and developmental processes. (C) 1996 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 00:36:00