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Titolo:
SURFACTANT-ASSOCIATED PROTEIN-A IS IMPORTANT FOR MAINTAINING SURFACTANT LARGE-AGGREGATE FORMS DURING SURFACE-AREA CYCLING
Autore:
VELDHUIZEN RAW; YAO LJ; HEARN SA; POSSMAYER F; LEWIS JF;
Indirizzi:
UNIV WESTERN ONTARIO,DEPT MED LONDON ON N6A 5A5 CANADA UNIV WESTERN ONTARIO,DEPT BIOCHEM LONDON ON N6A 5A5 CANADA UNIV WESTERN ONTARIO,DEPT PATHOL LONDON ON N6A 5A5 CANADA UNIV WESTERN ONTARIO,DEPT OBSTET & GYNAECOL LONDON ON N6A 5A5 CANADA UNIV WESTERN ONTARIO,MRC,GRP FETAL & NEONATAL HLTH & DEV LONDON ON N6A 5A5 CANADA
Titolo Testata:
Biochemical journal
, volume: 313, anno: 1996,
parte:, 3
pagine: 835 - 840
SICI:
0264-6021(1996)313:<835:SPIIFM>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PULMONARY SURFACTANT; ALVEOLAR SURFACTANT; EXTRACELLULAR METABOLISM; TUBULAR MYELIN; SP-B; SP-A; LUNG; SUBFRACTIONS; CONVERSION; SUBTYPES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
R.A.W. Veldhuizen et al., "SURFACTANT-ASSOCIATED PROTEIN-A IS IMPORTANT FOR MAINTAINING SURFACTANT LARGE-AGGREGATE FORMS DURING SURFACE-AREA CYCLING", Biochemical journal, 313, 1996, pp. 835-840

Abstract

Alveolar surfactant can be separated into two major subfractions, thelarge surfactant aggregates (LAs) and the small surfactant aggregates(SAs). The surface-active LAs are the metabolic precursors of the inactive SAs. This conversion of LAs into SAs can be studied in vitro using a technique called surface-area cycling, We have utilized this technique to examine the effect of trypsin on aggregate conversion. Our results show that trypsin increases the conversion of LAs into SAs in a concentration- and time-dependent manner. Immunoblot analysis revealedthat surfactant-associated Protein A (SP-A) was the main target of trypsin. To examine further the role of SP-A in aggregate conversion, wetested the effect of Ca2+ and mannan on this process. The absence of Ca2+ (1 mM EDTA) and the presence of mannan both increased the formation of SAs. Electron microscopy revealed that highly organized multilamellar and tubular myelin structures were present in samples that converted slowly to SAs. We concluded that SP-A is important for maintaining LA forms during surface-area cycling by stabilizing tubular myelin and multilamellar structures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 04:33:51