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Titolo:
IDENTIFICATION OF A TRANSCRIPTION FACTOR THAT BINDS TO THE S-BOX OF THE I-A-BETA GENE OF THE MAJOR HISTOCOMPATIBILITY COMPLEX
Autore:
CELADA A; GIL P; MCKERCHER SR; MAKI RA;
Indirizzi:
UNIV BARCELONA,FAC BIOL,DEPT FISIOL IMMUNOL,CAMPUS BELLVITGE E-08028 BARCELONA SPAIN UNIV BARCELONA,FUNDACIO AUGUST PI & SUMYER E-08028 BARCELONA SPAIN LA JOLLA CANC RES FDN LA JOLLA CA 92037
Titolo Testata:
Biochemical journal
, volume: 313, anno: 1996,
parte:, 3
pagine: 737 - 744
SICI:
0264-6021(1996)313:<737:IOATFT>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR NECROSIS FACTOR; MACROPHAGE CELL-LINE; CIS-ACTING SEQUENCES; DR-ALPHA GENE; INTERFERON-GAMMA; DNA-BINDING; GLUCOCORTICOID RECEPTOR; MAMMALIAN-CELLS; NUCLEAR FACTOR; Y-ELEMENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
60
Recensione:
Indirizzi per estratti:
Citazione:
A. Celada et al., "IDENTIFICATION OF A TRANSCRIPTION FACTOR THAT BINDS TO THE S-BOX OF THE I-A-BETA GENE OF THE MAJOR HISTOCOMPATIBILITY COMPLEX", Biochemical journal, 313, 1996, pp. 737-744

Abstract

Class II genes of the MHC show a striking homology upstream of the transcription start site that is composed of three conserved sequences (S, X and Y boxes, each separated by 15-20 bp). The presence of the S-box sequence in the mouse MHC class II gene I-A beta was examined for its influence on the expression of this gene. Deletion or mutation of the S box decreased the induction of chloramphenicol acetyltransferase (CAT) activity in B lymphocytes by 32%. In macrophages, deletion or mutation of the S box abolished interferon-gamma (IFN-gamma) inducibility of CAT activity. Using a gel-retardation assay, we have identified anuclear factor whose binding site overlaps the 7-mer conserved sequence of the S box. This factor is present in lymphocytes, macrophages, mastocytes and fibroblasts. Surprisingly, binding of this nuclear factor to DNA was induced by IFN-gamma in bone-marrow-derived macrophages, but not in macrophage-like cell lines. The binding site for this factor was defined by DNase I footprinting and partially purified by using an affinity column containing double-stranded oligonucleotides containing a sequence of the S box. A prominent protein of 43 kDa was found that bound specifically to the S-box sequence.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 19:46:15