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Titolo:
ENDOCYTOSIS AND INTRACELLULAR PROCESSING ACCOMPANYING TRANSFECTION MEDIATED BY CATIONIC LIPOSOMES
Autore:
FRIEND DS; PAPAHADJOPOULOS D; DEBS RJ;
Indirizzi:
CALIF PACIFIC MED CTR,2330 CLAY ST,STERN BLDG SAN FRANCISCO CA 94115 HARVARD UNIV,SCH MED,DEPT RHEUMATOL & IMMUNOL BOSTON MA 02115 UNIV CALIF SAN FRANCISCO,DEPT PATHOL SAN FRANCISCO CA 94143 UNIV CALIF SAN FRANCISCO,DEPT CELLULAR & MOLEC PHARMACOL SAN FRANCISCO CA 94143 UNIV CALIF SAN FRANCISCO,CANC RES INST SAN FRANCISCO CA 94143
Titolo Testata:
Biochimica et biophysica acta. Biomembranes
fascicolo: 1, volume: 1278, anno: 1996,
pagine: 41 - 50
SICI:
0005-2736(1996)1278:1<41:EAIPAT>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMALIAN-CELLS; PROTOPLAST FUSION; DNA-TRANSFECTION; MACROMOLECULES; EXPRESSION; EFFICIENCY; VESICLES; DELIVERY; INVIVO;
Keywords:
CATIONIC LIPOSOME; GENE TRANSFER; ENDOCYTOSIS; TRANSFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
D.S. Friend et al., "ENDOCYTOSIS AND INTRACELLULAR PROCESSING ACCOMPANYING TRANSFECTION MEDIATED BY CATIONIC LIPOSOMES", Biochimica et biophysica acta. Biomembranes, 1278(1), 1996, pp. 41-50

Abstract

Cationic liposomes mediate efficient transfection of mammalian cells,but the manner in which cells internalize and process cationic liposome-DNA complexes has not been well characterized. We exposed several cell types, including human and murine erythroleukemia cells, African green monkey kidney cells (CV-1), isolated rat alveolar type II cells and alveolar macrophages to DNA-cationic liposome complexes containing N-(1-2,3-dioleyloxypropyl)-N,N,N-triethylammonium (DOTMA) and Dioleylphosphatidylethanolamine (DOPE). The morphology of liposome-cell interactions was assessed by electron microscopy. Liposome preparations werecomplexed to colloidal gold particles or to both plasmid DNA and goldparticles. Cells treated with DOTMA liposome-DNA complexes demonstrated endocytosis of the liposome-DNA complexes in coated pits, which were seen in early endosomes, late endosomes, and lysosomes. In isolated alveolar type II cells, the gold-labelled DOTMA lipid apparently mixedwith the contents of lamellar bodies. In most cells, gold particles were dispersed throughout the cytoplasmic matrix. In a small proportionof CV-I and U937 cells, a membrane system resembling the endoplasmic reticulum developed within the nucleus. This novel structure was also present in nuclei after they were isolated from CV-1 cells and then mixed with DOTMA-containing liposomes. Membranes which form after exposure to DOTMA-containing liposomes were 10 nm in thickness as compared to the approx. 8 nm thickness of endogenous cellular membranes. Based on these morphologic observations, we propose that the main route of entry of cationic liposomes into cells is by endocytosis. In some instances, the endosomal compartment releases its cationic liposome-DNA contents into the cytoplasmic matrix. Occasionally, liposomes may enter the nucleus by fusion with the nuclear envelope, creating vesicular and reticular intranuclear membranes. It is not clear at present which, ifany of these morphological observations correlates with transfection mediated by cationic liposomes.

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Documento generato il 26/09/20 alle ore 12:03:55