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Titolo:
REGULATION OF SOMATOSTATIN GENE-EXPRESSION BY VERATRIDINE-INDUCED DEPOLARIZATION IN CULTURED FETAL CEREBROCORTICAL CELLS
Autore:
TOLON RM; SANCHEZFRANCO F; FERNANDEZ JL; LORENZO MJ; VAZQUEZ GF; CACICEDO L;
Indirizzi:
HOSP RAMON Y CAJAL,SERV ENDOCRINOL E-28034 MADRID SPAIN HOSP RAMON Y CAJAL,SERV ENDOCRINOL E-28034 MADRID SPAIN INST CARLOS III,CTR NACL INVEST CLIN MADRID SPAIN
Titolo Testata:
Molecular brain research
fascicolo: 1-2, volume: 35, anno: 1996,
pagine: 103 - 110
SICI:
0169-328X(1996)35:1-2<103:ROSGBV>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROENKEPHALIN MESSENGER-RNA; CEREBRAL CORTICAL-CELLS; RAT-BRAIN-CELLS; CHROMAFFIN CELLS; MEMBRANE DEPOLARIZATION; HIPPOCAMPAL-NEURONS; PROTEIN-KINASE; CALCIUM; RELEASE; TRANSCRIPTION;
Keywords:
VERATRIDINE; VERATRIDINE-INDUCED SS GENE EXPRESSION; SS MESSENGER-RNA; BRAIN SOMATOSTATIN; LONG-TERM DEPOLARIZATION; SHORT-TERM DEPOLARIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
R.M. Tolon et al., "REGULATION OF SOMATOSTATIN GENE-EXPRESSION BY VERATRIDINE-INDUCED DEPOLARIZATION IN CULTURED FETAL CEREBROCORTICAL CELLS", Molecular brain research, 35(1-2), 1996, pp. 103-110

Abstract

The stimulatory effect of veratridine (VTD) depolarization upon somatostatin mRNA (SS mRNA) levels in primary cultures of fetal cerebrocortical cells was analyzed. Depolarizing stimuli, such as 100 mu M VTD exposure for 30 min, elicited an increase in immunoreactive somatostatin(IR-SS) release to the media without affecting SS mRNA levels. These levels increased when exposure to depolarization stimuli was prolongedup to 3 or more hours. At this time, veratridine acted as a secretagogue, stimulating somatostatin secretion, but was also effective in stimulating somatostatin mRNA levels. These changes were blunted by the Na+ channel blockade tetrodotoxin (TTX), and partially abolished by theCa2+ channel antagonist, verapamil (VPM). To study whether VTD may affect mRNA stability we determine the rate of disappearance of SS mRNA after inhibition of transcription by actinomycin D and demonstrated that VTD stimulation did not stabilize the SS mRNA. These results indicate that the induction of SS mRNA expression by VTD involves the modulation of Ca2+ and Na+ channels. The time course study confirmed that the VTD-induced SS mRNA accumulation is time-dependent, and requires a prolonged exposure to stimulate SS gene expression. VTD stimulation does not modify the SS mRNA rate of degradation.

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Documento generato il 31/10/20 alle ore 00:34:26