Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A NEW METHOD FOR ISOLATION OF CARDIAC MYOCYTES BY PERCUTANEOUS ENDOMYOCARDIAL BIOPSY
Autore:
ABELA GS; COHEN MC; CORNELIUS JG;
Indirizzi:
MICHIGAN STATE UNIV,DEPT MED,DIV CARDIOL,B208 CLIN CTR E LANSING MI 48824 HARVARD UNIV,DEACONESS HOSP,SCH MED,DEPT MED,DIV CARDIOVASC BOSTON MA00000 UNIV FLORIDA,DIV CARDIOL GAINESVILLE FL 00000 UNIV FLORIDA,DEPT PATHOL & LAB MED GAINESVILLE FL 00000
Titolo Testata:
Catheterization and cardiovascular diagnosis
fascicolo: 2, volume: 37, anno: 1996,
pagine: 227 - 230
SICI:
0098-6569(1996)37:2<227:ANMFIO>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN VENTRICULAR TISSUE; HEART-CELLS; SINGLE ATRIAL; CALCIUM;
Keywords:
MYOPATHY; MYOCARDIUM; MYOCYTES; MYOCARDITIS; BIOPSY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
G.S. Abela et al., "A NEW METHOD FOR ISOLATION OF CARDIAC MYOCYTES BY PERCUTANEOUS ENDOMYOCARDIAL BIOPSY", Catheterization and cardiovascular diagnosis, 37(2), 1996, pp. 227-230

Abstract

The objective of this study was to demonstrate the feasibility of isolating viable canine cardiac myocytes from percutaneous right ventricular endomyocardial biopsy specimens, Although histologic data can be obtained from percutaneous endomyocardial biopsies, this approach has not been used as a source of viable cells for evaluating pathological conditions, Study of isolated viable myocytes may provide insight into the electrical, biochemical, and physiologic functions of the heart. Using a standard 8F sheath, a 5F bioptome was introduced via the right femoral vein and advanced to the right ventricle, where 85 biopsies were obtained from 8 mongrel dogs. An average of six biopsy specimens were pooled for processing to provide adequate tissue substrate. This resulted in 14 groups of specimens which were then processed to isolate individual myocytes. Viable myocytes were striated, rod-shaped, and excluded trypan blue dye. Nonviable myocytes were rounded, had no cross-striations, and did stain with trypan blue. Partially-injured myocytescontracted spontaneously and had a region of loss of cross-striations. The average number of viable cells recovered per group of pooled specimens was 1.8 x 10(4) (1.8 x 10(3) cells/mg of tissue). The greatest yield of viable myocytes recovered was 8.0 x 10(4), which represented a viability of 90% by trypan blue dye exclusion and morphological criteria. Percutaneous right ventricular endomyocardial biopsy is a novel method for obtaining viable cardiac myocytes, Its feasibility and utility in humans warrant further investigation. (C) 1996 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 18:40:05