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Titolo:
ESTABLISHMENT AND CHARACTERIZATION OF A MURINE CD4(-CELL LINE AND CLONE THAT INDUCE EXPERIMENTAL AUTOIMMUNE UVEORETINITIS IN B10.A MICE() T)
Autore:
RIZZO LV; SILVER P; WIGGERT B; HAKIM F; GAZZINELLI RT; CHAN CC; CASPI RR;
Indirizzi:
NEI,NIH,IMMUNOL LAB,BLDG 10,ROOM 10N202 BETHESDA MD 20892 NCI,NIH BETHESDA MD 20892 NIAID,NIH BETHESDA MD 20892 UNIV FED MINAS GERAIS,DEPT BIOCHEM & IMMUNOL BELO HORIZONT MG BRAZIL
Titolo Testata:
The Journal of immunology
fascicolo: 4, volume: 156, anno: 1996,
pagine: 1654 - 1660
SICI:
0022-1767(1996)156:4<1654:EACOAM>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
RETINOID-BINDING PROTEIN; BETA GENE USAGE; MULTIPLE-SCLEROSIS; REACTIVE ARTHRITIS; RECEPTOR; TH1; ANTIGEN; ALPHA; EAU; UVEITIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
L.V. Rizzo et al., "ESTABLISHMENT AND CHARACTERIZATION OF A MURINE CD4(-CELL LINE AND CLONE THAT INDUCE EXPERIMENTAL AUTOIMMUNE UVEORETINITIS IN B10.A MICE() T)", The Journal of immunology, 156(4), 1996, pp. 1654-1660

Abstract

B10.A mice develop experimental autoimmune uveoretinitis after activeimmunization with the interphotoreceptor retinoid-binding protein (IRBP), CD4(+) T cells play an important role in the development of the disease, In this study we have isolated and characterized a CD4(+) T cell line and a T cell clone that induce experimental autoimmune uveoretinitis when transferred into naive B10.A mice, The cell line was isolated from draining lymph nodes of IRBP-immunized animals by repeated cycles of IRBP stimulation, The line was shown to be pathogenic after 4 rounds of in vitro stimulation with IRBP at 5 x 10(6) cells/mouse, A Tcell clone derived from this line by limiting dilution was shown to be pathogenic when the same number of cells was injected; incidence andseverity of disease, however, were much lower, After 16 rounds of IRBP-specific stimulation the cell line was pathogenic at 10(5) cells/mouse. Analysis of the VP repertoire revealed that at this point the linewas mostly composed of V beta 8.2- and V beta 6-positive cells (>80% of the population), The uveitogenic clone expressed V beta 8.2. Both the T cell line and the clone elaborated an unrestricted lymphokine profile in vitro, However, when these cells were adoptively transferred into naive recipients, mRNA isolated from the uveitic retina showed only Th1 type cytokines, These data help to characterize the nature of pathogenic cells involved in ocular autoimmunity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 00:50:59