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Titolo:
DERMAL MICROVASCULAR ENDOTHELIAL-CELLS EXPRESS CD32 RECEPTORS IN-VIVOAND IN-VITRO
Autore:
GROGER M; SARMAY G; FIEBIGER E; WOLFF K; PETZELBAUER P;
Indirizzi:
UNIV VIENNA,SCH MED,DEPT DERMATOL,WAHRINGER GURTEL 18-20 A-1090 VIENNA AUSTRIA UNIV VIENNA,SCH MED,DEPT DERMATOL A-1090 VIENNA AUSTRIA SFI,VIENNA INT RES CTR,IMMUNOREGULAT LAB VIENNA AUSTRIA
Titolo Testata:
The Journal of immunology
fascicolo: 4, volume: 156, anno: 1996,
pagine: 1549 - 1556
SICI:
0022-1767(1996)156:4<1549:DMEECR>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
FC-GAMMA-RIIA; IMMUNE THROMBOCYTOPENIC PURPURA; DIFFERENTIAL EXPRESSION; IGG; HETEROGENEITY; COMPLEXES; FRAGMENTS; POLYMORPHISM; PHAGOCYTOSIS; LYMPHOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
M. Groger et al., "DERMAL MICROVASCULAR ENDOTHELIAL-CELLS EXPRESS CD32 RECEPTORS IN-VIVOAND IN-VITRO", The Journal of immunology, 156(4), 1996, pp. 1549-1556

Abstract

Immune complexes are thought to be the major cause of cutaneous necrotizing vasculitis, but the mechanism of immune complex targeting to specific vessels is largely unknown. In myelomonocytic cells, immune complex binding and receptor-mediated endocytosis are mediated by Fc gamma R. We asked whether dermal microvascular endothelial cells (DMEC) express Fc gamma Rs. In cryostat sections of normal human skin, mAb IV.3or AT10, both recognizing CD32 (Fc gamma RII), localizes to the luminal surface of DMEC of the superficial but not of the deep vascular plexus. All DMEC do not express CD16 (Fc gamma RIII) or CD64 (Fc gamma RI) molecules. Adult skin-derived DMEC in culture express CD32 (Fc gammaRII) molecules, as measured by FAGS, but are negative for CD16 or CD64. HUVEC, tested for comparison, do not express CD16, 32, or 64 proteins. Ey reverse-transcriptase PCR and subsequent Southern blot analysis, the isoform of the CD32 molecule expressed on DMEC is determined as Fc gamma RIIa. HUVEC do not contain Fc gamma RIIa or Fc gamma RIIb mRNA. In DMEC, Fc gamma RIIa cross-linking results in immediate intracellular free Ca2+ ([Ca2+](i)) concentration fluxes and in rapid internalization of the occupied receptors. We conclude that DMEC are equipped with fully functional Fc gamma RIIa molecules.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:36:53