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Titolo:
MYRISTATE EXCHANGE ON THE TRYPANOSOMA-BRUCEI VARIANT SURFACE GLYCOPROTEIN
Autore:
BUXBAUM LU; MILNE KG; WERBOVETZ KA; ENGLUND PT;
Indirizzi:
JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM BALTIMORE MD 21205 JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM BALTIMORE MD 21205
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 3, volume: 93, anno: 1996,
pagine: 1178 - 1183
SICI:
0027-8424(1996)93:3<1178:MEOTTV>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEMBRANE ANCHOR; BIOSYNTHESIS; ACID; GLYCAN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
L.U. Buxbaum et al., "MYRISTATE EXCHANGE ON THE TRYPANOSOMA-BRUCEI VARIANT SURFACE GLYCOPROTEIN", Proceedings of the National Academy of Sciences of the United Statesof America, 93(3), 1996, pp. 1178-1183

Abstract

The glycosyl-phosphatidylinositol (GPI) anchor of the Trypanosoma brucei variant surface glycoprotein (VSG) is unique in having exclusivelymyristate as its fatty acid component, We previously demonstrated that the myristate specificity is the result of two independent pathways,First, the newly synthesized free GPI, which is not myristoylated, undergoes fatty acid remodeling to replace both its fatty acids with myristate, Second, the myristoylated precursor, glycolipid A, undergoes amyristate exchange reaction, detected by the replacement of unlabeledmyristate by [H-3]myristate. Remodeling and exchange have different enzymatic properties and apparently occur in different subcellular compartments, We now demonstrate that the GPI anchor linked to VSG is the major substrate for myristate exchange, VSG can be efficiently labeledwith [H-3]myristate by exchange in the presence of cycloheximide, an inhibitor that prevents new VSG synthesis and thus anchor addition to protein, Not only is newly synthesized VSG subject to exchange, but mature VSG, possibly recycling from the cell surface, also undergoes myristate exchange.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 23:39:35