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Titolo:
GLUCOCORTICOIDS REGULATE NHE-3 TRANSCRIPTION IN OKP CELLS
Autore:
BAUM M; AMEMIYA M; DWARAKANATH V; ALPERN RJ; MOE OW;
Indirizzi:
UNIV TEXAS,SW MED CTR,DEPT PEDIAT,5323 HARRY HINES BLVD DALLAS TX 75235 UNIV TEXAS,SW MED CTR,DEPT INTERNAL MED DALLAS TX 75235 DEPT VET AFFAIRS MED CTR DALLAS TX 75235
Titolo Testata:
American journal of physiology. Renal, fluid and electrolyte physiology
fascicolo: 1, volume: 39, anno: 1996,
pagine: 164 - 169
SICI:
0363-6127(1996)39:1<164:GRNTIO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
RENAL BRUSH-BORDER; NA+-H+ EXCHANGER; HORMONE MESSENGER-RNA; PITUITARY-TUMOR CELLS; MEMBRANE-VESICLES; RABBIT KIDNEY; AMILORIDE; ISOFORM; DEXAMETHASONE; STIMULATION;
Keywords:
DEXAMETHASONE; SODIUM/PROTON EXCHANGER; PROXIMAL TUBULE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
M. Baum et al., "GLUCOCORTICOIDS REGULATE NHE-3 TRANSCRIPTION IN OKP CELLS", American journal of physiology. Renal, fluid and electrolyte physiology, 39(1), 1996, pp. 164-169

Abstract

KP cells express NHE-3, an amiloride-resistant Na+/H+ antiporter, which is likely an isoform responsible for apical proton secretion by theproximal tubule. We have previously shown that an amiloride-resistantNa+/H+ antiporter in OKP cells is regulated by dexamethasone, a synthetic glucocorticoid. The purpose of the present study was to examine the mechanism for the glucocorticoid-mediated increase in Na+/H+ antiporter activity. Incubation of OKP cells with 10(-6) M dexamethasone resulted in a two- to threefold increase in NHE-3 mRNA abundance. This increase was seen after 4 h of incubation with dexamethasone, a time course similar to that found for Na+/H+ antiporter activity. To examine the mechanism for the increase in NHE-3 mRNA abundance, mRNA half-life and in vitro transcription experiments were performed. NHE-3 mRNA had a half-life of 8 h in control and dexamethasone-treated cells. The rate of in vitro transcription was 1.8-fold greater when OKP cells were treated with dexamethasone. These data suggest that the glucocorticoid-mediated increase in Na+/H+ antiporter activity is due to an increase in NHE-3 gene transcription.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 08:07:52