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Titolo:
CHOLESTEROL AND PHOSPHOLIPID LEVELS OF WASHED AND PERCOLL GRADIENT CENTRIFUGED MOUSE SPERM - PRESENCE OF LIPIDS POSSESSING INHIBITORY EFFECTS ON SPERM MOTILITY
Autore:
TANPHAICHITR N; ZHENG YS; KATES M; ABDULLAH N; CHAN A;
Indirizzi:
OTTAWA CIVIC HOSP,LOEB MED RES INST,REPROD BIOL UNIT,HUMAN IN VITRO FERTILIZAT LABS OTTAWA ON K1Y 4E9 CANADA UNIV OTTAWA,DEPT OBSTET & GYNECOL OTTAWA ON CANADA UNIV OTTAWA,DEPT BIOCHEM OTTAWA ON CANADA
Titolo Testata:
Molecular reproduction and development
fascicolo: 2, volume: 43, anno: 1996,
pagine: 187 - 195
SICI:
1040-452X(1996)43:2<187:CAPLOW>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALBUMIN-MEDIATED CHANGES; EPIDIDYMAL MATURATION; PLASMA-MEMBRANE; RAT SPERM; INVITRO CAPACITATION; FATTY-ACIDS; SPERMATOZOA; FERTILIZATION; PROTEIN; BOAR;
Keywords:
SPERM LIPIDS; SPERM CHOLESTEROL; SPERM PHOSPHOLIPIDS; SPERM DNA; SPERM MOTILITY; SPERM PREPARATION; SPERM CAPACITATION; SPERM AGGREGATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
N. Tanphaichitr et al., "CHOLESTEROL AND PHOSPHOLIPID LEVELS OF WASHED AND PERCOLL GRADIENT CENTRIFUGED MOUSE SPERM - PRESENCE OF LIPIDS POSSESSING INHIBITORY EFFECTS ON SPERM MOTILITY", Molecular reproduction and development, 43(2), 1996, pp. 187-195

Abstract

Levels of DNA, cholesterol, and phospholipids of mouse caudal epididymal and vas deferens sperm that were processed through simple washing and Percoll gradient centrifugation were measured. The DNA and cholesterol contents of washed sperm and Percoll gradient centrifuged (PGC) sperm (DNA = 3.6 +/- 0.3 pg/sperm and 3.4 +/- 0.3 pg/sperm, respec tively; cholesterol = 0.219 +/- 0.057 nmole/mu g DNA and 0.224 +/- 0.030 nmole/mu g DNA, respectively, for washed and PGC sperm) were not significantly different from each other; however, the phospholipid level of PGC sperm was only one half of that of washed sperm (0.315 +/- 0.071 nmole/mu g DNA versus 0.720 +/- 0.075 nmole/mu g DNA, respectively). The presence of 0.3% bovine serum albumin (BSA) in the culture medium used in sperm washing did not change the cholesterol and phospholipid contents of washed sperm. Similarly, the cholesterol and phospholipid levels of washed sperm and PGC sperm that were further incubated in BSA-containing medium for 30 min remained the same. Interestingly, substantial amounts of lipids, as determined by the cholesterol and phospholipid levels, were released into the supernatants of the sperm washes, and sperm needed to be washed at least twice to ensure their stable levels of cholesterol and phospholipids. The lipid mixture in the first sperm wash supernatant was shown to have inhibitory effects on PGC sperm motility. (C) 1996 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 03:01:42