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Titolo:
IS THE LACK OF PROTEIN F1 GAP-43 MESSENGER-RNA IN GRANULE CELLS TARGET-DEPENDENT/
Autore:
MEBERG PJ; JARRARD LE; ROUTTENBERG A;
Indirizzi:
NORTHWESTERN UNIV,CRESAP NEUROSCI LAB,2021 SHERIDAN RD EVANSTON IL 60208 NORTHWESTERN UNIV,CRESAP NEUROSCI LAB EVANSTON IL 60208 WASHINGTON & LEE UNIV,DEPT PSYCHOL LEXINGTON VA 24450
Titolo Testata:
Brain research
fascicolo: 2, volume: 706, anno: 1996,
pagine: 217 - 226
SICI:
0006-8993(1996)706:2<217:ITLOPF>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
KINASE-C SUBSTRATE; FIBRILLARY ACIDIC PROTEIN; MESSENGER-RNA EXPRESSION; LONG-TERM POTENTIATION; HIPPOCAMPAL-FORMATION; SYNAPTIC ENHANCEMENT; GENE-EXPRESSION; GANGLION-CELLS; GAP-43; RAT;
Keywords:
GAP-43; KAINATE; ASTROCYTE; IBOTENIC ACID; GLIAL FIBRILLARY ACIDIC PROTEIN (GFAP); HIPPOCAMPUS; DENTATE GYRUS; CA3;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
P.J. Meberg et al., "IS THE LACK OF PROTEIN F1 GAP-43 MESSENGER-RNA IN GRANULE CELLS TARGET-DEPENDENT/", Brain research, 706(2), 1996, pp. 217-226

Abstract

Protein F1/GAP-43 is differentially expressed in brain with high levels present in regions associated with memory functions. However, in hippocampus the granule cells lack F1/GAP-43 expression. To determine ifthis lack of expression is due to inhibitory signals from the target cells, we selectively destroyed CA3 pyramidal cells unilaterally usingmicroinjections of excitotoxins. Kainate lesions induced F1/GAP-43 mRNA expression bilaterally in granule cells at 24 h post-injection. Since the induction contralateral to the lesion was not due to loss of target cells, that induction may be ascribed to consequences of seizure activity. However, F1/GAP-43 mRNA hybridization decreased by 3 d post-lesion and was at background levels by 6 d, indicating that the lack of F1/GAP-43 expression in granule cells is restored despite a lack of target neurons. Unilateral lesions of CA3 cells using ibotenate, whichare not as complete as kainate but do not cause seizures, did not induce F1/GAP-43 mRNA in granule cells on either the contralateral or, in4 of 5 cases, the ipsilateral side. Taken together, these data suggest that the CA3 target is not essential for the absence of F1/GAP-43 expression in granule cells. To compare the extent of damage caused by the lesions, we investigated the location of astrocytes undergoing reactive gliosis, employing as a reporter glial fibrillary acidic protein (GFAP) gene expression. After both kainate and ibotenate injections GFAP hybridization increased in the lesioned area as well as in the contralateral hippocampus. These results indicate that injections of kainate, and possibly ibotenate to a lesser extent, may affect behavior notonly by damaging cells at the injection site, but also by altering gene expression in cells at distant sites.

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Documento generato il 30/03/20 alle ore 13:23:58