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Titolo:
MOLECULAR ANALYSIS OF RAT EMBRYO CELL TRANSFORMANTS INDUCED BY ALPHA-PARTICLES
Autore:
ENDLICH B; SALAVATI R; ZHANG J; WEISS H; LING CC;
Indirizzi:
MEM SLOAN KETTERING CANC CTR,RADIAT BIOPHYS LAB NEW YORK NY 10021
Titolo Testata:
International journal of radiation biology
fascicolo: 6, volume: 64, anno: 1993,
pagine: 715 - 726
SICI:
0955-3002(1993)64:6<715:MAOREC>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
RADIATION-INDUCED TRANSFORMATION; C-MYC; K-RAS; IONIZING-RADIATION; GAMMA-IRRADIATION; ENHANCED EXPRESSION; N-METHYLNITROSOUREA; THYMIC LYMPHOMAS; MUTATIONS; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
B. Endlich et al., "MOLECULAR ANALYSIS OF RAT EMBRYO CELL TRANSFORMANTS INDUCED BY ALPHA-PARTICLES", International journal of radiation biology, 64(6), 1993, pp. 715-726

Abstract

An immortal cell line was established by transfecting a myc oncogene into rat embryo cells (REC:myc)1. This cell line was diploid, contact inhibited and grew well in culture. Exposure to a single 200 cGy dose of 6 MeV alpha-particles transformed these cells with a frequency of focus formation of approximately 3.6 x 10(-4) compared with a transformation frequency of < 7.8 x 10(-6) for primary cultures of REC. Isolates of alpha-particle-induced REC:myc (REC:myc:alpha) foci displayed anchorage-independent growth in soft agar and were tumourigenic in nude mice. Molecular studies demonstrated no alteration of gene structure orexpression of the transfected or of the endogenous c-myc genes. Similarly, there was no alteration of the structure of Ha-ras, Ki-ras, or N-ras. The expression of Ha-ras, Ki-ras, N-ras and raf was not altered significantly. Assay for dominant oncogenes via DNA-mediated gene transfer into NIH3T3 cells was positive for nine of 13 REC:myc:alpha transformants. All NIH3T3 isolates contained bands hybridizing to rat repetitive DNA. NIH3T3 transformants from a tertiary round of transfection were analysed by Southern blot analysis for the presence of Ki-ras, N-ras, raf, trk, abl, fms, src, mos, fos, sis, fps, erbA, erbB or neu oncogenes of REC origin, and none were detected. Tertiary NIH3T3 transformants from three REC:myc:alpha transformants contained bands corresponding to Ha-ras but no point mutations were identified at the known hotspots of exons 1 or 2 of the donor REC:myc:alpha transformants. The inactivation of the tumour suppressor genes Rb, and p53, and the antimetastasis gene, nm23, was evaluated by Southern and Northern hybridization analysis. Southern blots demonstrated that at least one allele of Rb, p53 and nm23 was present and no large scale structural changes were detected. No expression of Rb or p53 was detected in REC:myc or the alpha-particle-induced REC:myc transformants. The expression of nm23 was not altered in the transformed cell lines. While the analysis of the role of tumour suppressor gene inactivation in radiation-induced cell transformation is only in the initial stages, the results of DNA-mediated gene transfer into NIH3T3 cells suggest that unidentified dominant oncogenes are associated with alpha-particle-induced transformationin vitro.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 09:38:22