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Titolo:
DISTRIBUTION AND SITES OF SYNTHESIS OF NTT4, AN ORPHAN MEMBER OF THE NA+ CL--DEPENDENT NEUROTRANSMITTER TRANSPORTER FAMILY, IN THE RAT CNS/
Autore:
LUQUE JM; JURSKY F; NELSON N; RICHARDS JG;
Indirizzi:
UNIV BASEL,DEPT PHARMACOL,KLINGELBERGSTR 70 CH-4056 BASEL SWITZERLAND F HOFFMANN LA ROCHE & CO LTD,PRECLIN RES,DIV PHARMA CH-4002 BASEL SWITZERLAND ROCHE INST MOLEC BIOL NUTLEY NJ 07110
Titolo Testata:
European journal of neuroscience
fascicolo: 1, volume: 8, anno: 1996,
pagine: 127 - 137
SICI:
0953-816X(1996)8:1<127:DASOSO>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
SENSITIVE DOPAMINE TRANSPORTER; BRAIN GABA TRANSPORTER; GLYCINE TRANSPORTER; MOUSE-BRAIN; FUNCTIONAL-CHARACTERIZATION; SEROTONIN TRANSPORTER; MOLECULAR-CLONING; SPLICE VARIANTS; NMDA RECEPTOR; BETA-ALANINE;
Keywords:
CARRIER; GLUTAMATE/GLYCINE; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
60
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Luque et al., "DISTRIBUTION AND SITES OF SYNTHESIS OF NTT4, AN ORPHAN MEMBER OF THE NA+ CL--DEPENDENT NEUROTRANSMITTER TRANSPORTER FAMILY, IN THE RAT CNS/", European journal of neuroscience, 8(1), 1996, pp. 127-137

Abstract

The distribution and sites of synthesis in rat CNS of NTT4, a novel orphan member of the Na+/Cl--dependent neurotransmitter transporter family, were determined by immunohistochemistry and hybridization histochemistry. Antibodies raised against recombinant fusion proteins, corresponding to residues of NTT4, and S-35-labelled oligodeoxyribonucleotide probes, were used to delineate the cellular distribution of the transporter at the protein and mRNA levels. High levels of immunoreactivity (mainly in the neuropil) were found in the olfactory bulb, cerebral cortex, striatum, hippocampus, thalamus, substantia nigra, pontine nuclei, cerebellum and spinal cord. The lowest levels were associated with the lateral hypothalamic area and deep mesencephalic nuclei. In situhybridization signals correlated well with the immunoreactivity, and demonstrated a widespread distribution of NTT4 transcripts exclusivelyin neurons. NTT4 transcripts appeared widely codistributed with the N-methyl-D-aspartate receptor subunit 1 (1-4b), i.e. spliced variants characterized by a common 5' 63 bp insertion. These results indicate that the transporter was associated with neuronal processes in specific glutamate innervated CNS regions. Although the substrate transported by NTT4 remains unknown, our findings suggest a possible role for this carrier protein in glutamate/glycine neurotransmission.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 23:52:20