Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
HUMAN CENTRAL NEUROCYTOMA CELLS SHOW NEURONAL PHYSIOLOGICAL-PROPERTIES IN-VITRO
Autore:
PATT S; SCHMIDT H; LABRAKAKIS C; WEYDT P; FRITSCH M; CERVOSNAVARRO J; KETTENMANN H;
Indirizzi:
FREE UNIV BERLIN,BENJAMIN FRANKLIN CLIN CTR,INST NEUROPATHOL,HINDENBURGDAMM 30 D-12200 BERLIN GERMANY MAX DELBRUCK CTR MOLEC MED,DEPT CELLULAR NEUROSCI BERLIN GERMANY MAX DELBRUCK CTR MOLEC MED,DEPT DEV NEUROBIOL BERLIN GERMANY CLIN CTR BERLIN BUCH,DEPT NEUROSURG BERLIN GERMANY
Titolo Testata:
Acta Neuropathologica
fascicolo: 2, volume: 91, anno: 1996,
pagine: 209 - 214
SICI:
0001-6322(1996)91:2<209:HCNCSN>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIFFERENTIATION; EXPRESSION;
Keywords:
HUMAN CENTRAL NEUROCYTOMA; SYNAPTOPHYSIN; GLIAL FIBRILLARY ACIDIC PROTEIN; PATCH CLAMP; SODIUM CHANNEL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
S. Patt et al., "HUMAN CENTRAL NEUROCYTOMA CELLS SHOW NEURONAL PHYSIOLOGICAL-PROPERTIES IN-VITRO", Acta Neuropathologica, 91(2), 1996, pp. 209-214

Abstract

Central neurocytoma is a rare brain tumor composed of small round synaptophysin-positive cells, suggesting a neuronal origin of these tumorcells. Glial properties are inferred, however, from the observation that the tumor cells exhibit a strong morphological similarity to oligodendroglioma cells and show an astrocytic differentiation in vitro. Totest for neuronal or glial physiological properties, we studied cultured neurocytoma cells derived from a surgical specimen from a 44-year-old man, employing the patch-clamp technique. Early primary cultures were composed of morphologically unique bi- or multipolar cells which were positive for synaptophysin and negative for the astrocyte marker glial fibrillary acidic protein. In the majority of these cells, whole-cell membrane current recordings revealed physiological properties of neurons, i.e., a high density of Na+ currents, the capacity to generate action potentials, and the expression of ionotropic neurotransmitterreceptors. Metabotropic neurotransmitter receptors could be demonstrated by Ca2+ imaging techniques. The remaining bi- or multipolar cells and almost all cells in later culture stages and in vitro passage lacked these neuronal properties and showed physiological features characteristic of glial cells. We conclude that the major population of neurocytoma cells shows physiological properties of neurons and that with time in culture this population is replaced by electrically passive cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 00:58:42