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Titolo:
ISOLATION AND CHARACTERIZATION OF KASUMI-1 HUMAN MYELOID-LEUKEMIA CELL-LINE RESISTANT TO TUMOR-NECROSIS-FACTOR ALPHA-INDUCED APOPTOSIS
Autore:
IDO M; HAYASHI K; KATO S; OGAWA H; KOMADA Y; ZHAU YW; ZHANG XL; SAKURAI M; SUZUKI K;
Indirizzi:
MIE UNIV,SCH MED,DEPT MOLEC PATHOBIOL,2-174 EDOBASHI TSU MIE 514 JAPAN MIE UNIV,SCH MED,DEPT PEDIAT TSU MIE 514 JAPAN
Titolo Testata:
British Journal of Cancer
fascicolo: 3, volume: 73, anno: 1996,
pagine: 360 - 365
SICI:
0007-0920(1996)73:3<360:IACOKH>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLECULAR-CLONING; MAP KINASE; RECEPTOR; TRANSLOCATION; ACTIVATION; EXPRESSION; LEUKEMIA; PROTEINS; CDNA; TNF;
Keywords:
TUMOR NECROSIS FACTOR; APOPTOSIS; PROTEIN KINASE; KASUMI-1 HUMAN MYELOID LEUKEMIA CELL LINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
M. Ido et al., "ISOLATION AND CHARACTERIZATION OF KASUMI-1 HUMAN MYELOID-LEUKEMIA CELL-LINE RESISTANT TO TUMOR-NECROSIS-FACTOR ALPHA-INDUCED APOPTOSIS", British Journal of Cancer, 73(3), 1996, pp. 360-365

Abstract

Tumour necrosis factor (TNF)-alpha induces apoptosis in a human acutemyeloid leukaemia cell line, Kasumi-1. To examine the role of proteinphosphorylation in signal transduction of TNF-alpha-induced apoptosis, a variant cell line resistant to TNF-alpha was established by an intermittent challenge of Kasumi-1 cells with increasing concentrations of TNF-alpha for 6 months. The mechanism of resistance to TNF-alpha appears to be in the post-receptor pathway because expression of p55 TNF receptor in the variant cells is increased compared with that of the parental Kasumi-1 cells. In renaturation assays, TNF-alpha induced a rapid activation of different protein kinases of different molecular weights, including the 50 kDa protein kinase (PK50) followed by the 35 kDa protein kinase (PK35), in the parental Kasumi-1 cells. The dose-response of TNF-alpha required to activate PK50 and PK35 was closely related to concentrations of TNF-alpha that induced apoptosis. Treatment ofKasumi-1 cells with ceramide also activated PK35. In TNF-resistant variant cells, activation of PK35 in response to TNF-alpha or ceramide was practically nil. These findings suggest that activation of PK35 through the ceramide pathway may play an important role in signal transduction of TNF-alpha in the Kasumi-1 cell line, while the decreased activation of PK35 may explain the insensitivity of the variant cells towards TNF-alpha.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 11:52:07