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Titolo:
ISOLATION OF PEPSIN-RESISTANT LAMININ FRAGMENTS FROM HUMAN PLACENTA -EFFECT ON EPITHELIAL-CELLS CULTURED FROM THE KIDNEYS OF PATIENTS WITHAUTOSOMAL-DOMINANT POLYCYSTIC KIDNEY-DISEASE (ADPKD)
Autore:
SLADE MJ; POCSI I; KIRBY RB; JONES JK; GANZ SE; TAYLOR SA; PRICE RG;
Indirizzi:
UNIV LONDON KINGS COLL,DIV LIFE SCI,BIOCHEM SECT,CAMPDEN HILL RD LONDON W8 7AH ENGLAND UNIV LONDON KINGS COLL,DIV LIFE SCI,BIOCHEM SECT LONDON W8 7AH ENGLAND
Titolo Testata:
Biochimica et biophysica acta. Molecular cell research
fascicolo: 1, volume: 1310, anno: 1996,
pagine: 25 - 31
SICI:
0167-4889(1996)1310:1<25:IOPLFF>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
BASEMENT-MEMBRANES; A-CHAIN; PROTOONCOGENE EXPRESSION; CPK MOUSE; GLYCOPROTEIN; DOMAINS; GROWTH; LOCALIZATION; FIBROBLASTS; INVITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
M.J. Slade et al., "ISOLATION OF PEPSIN-RESISTANT LAMININ FRAGMENTS FROM HUMAN PLACENTA -EFFECT ON EPITHELIAL-CELLS CULTURED FROM THE KIDNEYS OF PATIENTS WITHAUTOSOMAL-DOMINANT POLYCYSTIC KIDNEY-DISEASE (ADPKD)", Biochimica et biophysica acta. Molecular cell research, 1310(1), 1996, pp. 25-31

Abstract

Laminin isolated from human placenta was subjected to prolonged pepsin digestion. Seven peptide fragments (designated N1 to N7) were separated by ion-exchange chromatography and gel filtration and characterised by SDS-polyacrylamide gel electrophoresis and immunoblotting. The molecular size of the laminin fragments varied from approx, 900 000 (N1)to 28 000 (N7). Epithelial cells obtained from normal kidneys and patients with autosomal dominant polycystic kidney disease (ADPKD) were cultured. The incorporation of [H-3]thymidine was measured over 96 h todetermine the effect of the addition of the different fragments and whole laminin from EHS tumour to the cells. The rate of growth of both normal and polycystic cells was increased in the presence of the laminin fragments but this effect was more pronounced in the ADPKD cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/10/20 alle ore 04:39:35