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Titolo:
CELLULAR EXPRESSION OF MESSENGER-RNAS ENCODING MONOAMINE-OXIDASE-A AND MONOAMINE-OXIDASE-B IN THE RAT CENTRAL-NERVOUS-SYSTEM
Autore:
LUQUE JM; KWAN SW; ABELL CW; DAPRADA M; RICHARDS JG;
Indirizzi:
UNIV BASEL,BIOCTR,DEPT PHARMACOL,KLINGELBERGSTR 70 CH-4056 BASEL SWITZERLAND F HOFFMANN LA ROCHE & CO LTD,DIV PHARMA CH-4002 BASEL SWITZERLAND UNIV TEXAS,COLL PHARM AUSTIN TX 78712 UNIV TEXAS,INST NEUROSCI AUSTIN TX 78712
Titolo Testata:
Journal of comparative neurology
fascicolo: 4, volume: 363, anno: 1995,
pagine: 665 - 680
SICI:
0021-9967(1995)363:4<665:CEOMEM>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMPROVED HISTOCHEMICAL METHOD; HUMAN BRAIN-STEM; CONTAINING NEURONS; TYROSINE-HYDROXYLASE; CAT HYPOTHALAMUS; BINDING-SITES; IMMUNOREACTIVE NEURONS; EXTRACELLULAR 5-HYDROXYTRYPTAMINE; POSTERIOR HYPOTHALAMUS; MESSENGER-RNA;
Keywords:
MAO-A; MAO-B; HYBRIDIZATION HISTOCHEMISTRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
98
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Luque et al., "CELLULAR EXPRESSION OF MESSENGER-RNAS ENCODING MONOAMINE-OXIDASE-A AND MONOAMINE-OXIDASE-B IN THE RAT CENTRAL-NERVOUS-SYSTEM", Journal of comparative neurology, 363(4), 1995, pp. 665-680

Abstract

Monoamine oxidases A and B (MAO-A and MAO-B) oxidatively deaminate neurotransmitter and xenobiotic amines. The cellular localization of these isoenzymes in the central nervous system (CNS) differs markedly andonly partly reflects the distribution of their presumed natural substrates. In the present study, by using in situ hybridization with S-35-labelled oligonucleotide probes, we examined the distribution of mRNAsencoding MAO-A and MAO-B in the rat CNS. Probes for tyrosine hydroxylase, histidine decarboxylase, and tryptophan hydroxylase mRNAs were used to demonstrate the catecholaminergic, histaminergic, or serotoninergic nature of some cell populations in adjacent sections. The radioligands [H-3]-Ro 41-1049 and [H-3]lazabemide (reversible and selective inhibitors of MAO-A and MAO-B, respectively) were used to reveal the protein distribution by enzyme radioautography. The distribution and abundance of transcripts for both isoenzymes in the tissues investigated differed markedly but, in general, correlated with the protein distribution. MAO-A mRNA and protein were most abundant in noradrenergic neurons. However, moderate levels of transcript expression and protein werealso detected in the serotoninergic neurons, and low but significant levels were detected in the dopaminergic neurons. An unexpectedly remarkable degree of hybridization signal was apparent in nonaminergic cell populations, e.g., in the cerebral cortices, the hippocampal formation (CA1-3, dentate gyrus), the cerebellar granule cell layer, and the spinal cord motoneurons. In contrast, MAO-B mRNA and protein were mostabundant in serotoninergic and histaminergic neurons, Bergmann glial cells, and circumventricular organs, including the ependyma. MAO-B transcripts were also weakly expressed in nonaminergic cells, e.g., in the hippocampal formation (CA1-2). A further nonneuronal localization ofMAO-B transcripts was also resolved, e.g., in the glia limitans, the olfactory nerve layer, and the cerebellar peduncle. These findings reveal further the potential of various cell populations to synthesize the isoenzymes, and homologous (aminergic) and heterologous (nonaminergic) patterns of expression as well as coexpression of MAO mRNAs are described. (C) 1995 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 13:38:39