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Titolo:
ACTIVATION AND REPRESSION OF TRANSCRIPTION AT 2 DIFFERENT PHAGE PHI-29 PROMOTERS ARE MEDIATED BY INTERACTION OF THE SAME RESIDUES OF REGULATORY PROTEIN P4 WITH RNA-POLYMERASE
Autore:
MONSALVE M; MENCIA M; ROJO F; SALAS M;
Indirizzi:
UNIV AUTONOMA MADRID,CSIC,CTR BIOL MOLEC SEVERO OCHOA,CANTO BLANCO,CAMPUS UNIV AUTONOMA MADRID E-28049 MADRID SPAIN UNIV AUTONOMA MADRID,CSIC,CTR BIOL MOLEC SEVERO OCHOA E-28049 MADRID SPAIN CSIC,CTR NACL BIOTECNOL E-28049 MADRID SPAIN
Titolo Testata:
EMBO journal
fascicolo: 2, volume: 15, anno: 1996,
pagine: 383 - 391
SICI:
0261-4189(1996)15:2<383:AAROTA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
INITIAL TRANSCRIBING COMPLEX; NUCLEIC-ACID INTERACTIONS; ESCHERICHIA-COLI; LAC REPRESSOR; DNA; BINDING; MECHANISM; INVITRO;
Keywords:
BACILLUS SUBTILIS; GENE EXPRESSION; PROMOTERS; PROTEIN-PROTEIN CONTACTS; TRANSCRIPTION REPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
M. Monsalve et al., "ACTIVATION AND REPRESSION OF TRANSCRIPTION AT 2 DIFFERENT PHAGE PHI-29 PROMOTERS ARE MEDIATED BY INTERACTION OF THE SAME RESIDUES OF REGULATORY PROTEIN P4 WITH RNA-POLYMERASE", EMBO journal, 15(2), 1996, pp. 383-391

Abstract

Phage Phi 29 regulatory protein p4 activates transcription from the late A3 promoter and represses the main early promoters, named A2b and A2c, Activation involves stabilization of RNA polymerase (RNAP) at theA3 promoter as a closed complex and is mediated by interaction between RNAP and a small domain of protein p4 in which residue Arg120 plays an essential role, We show that protein p4 represses the A2c promoter by binding to DNA immediately upstream from RNAP in a way that does not hinder RNAP binding; rather, the two proteins bind cooperatively to DNA, In the presence of protein p4, RNAP can form an initiated complexat the A2c promoter that generates short abortive transcripts, but cannot leave the promoter, Mutation of protein p4 residue Arg120, which relieves the contact between the two proteins, leads to a loss of repression, Therefore, the contact between protein p4 and RNAP through theprotein p4 domain containing Arg120 can activate or repress transcription, depending on the promoter, The relative position of protein p4 and RNAP, which is different at each promoter, together with the distinct characteristics of the two promoters, may determine whether proteinp4 activates or represses transcription.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 18:20:29