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Titolo:
EFFECTS OF INJECTING CALCIUM-CHLORIDE INTO IN VITRO-MATURED PORCINE OOCYTES
Autore:
MACHATY Z; FUNAHASHI H; MAYES MA; DAY BN; PRATHER RS;
Indirizzi:
UNIV MISSOURI,DEPT ANIM SCI,162 ANIM SCI RES CTR COLUMBIA MO 65211 UNIV MISSOURI,DEPT ANIM SCI COLUMBIA MO 65211
Titolo Testata:
Biology of reproduction
fascicolo: 2, volume: 54, anno: 1996,
pagine: 316 - 322
SICI:
0006-3363(1996)54:2<316:EOICII>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR; SEA-URCHIN EGGS; MOUSE OOCYTES; PARTHENOGENETIC DEVELOPMENT; ENDOPLASMIC-RETICULUM; MEIOTIC MATURATION; HISTONE-H1 KINASE; CORTICAL GRANULES; SKELETAL-MUSCLE; XENOPUS EGGS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
66
Recensione:
Indirizzi per estratti:
Citazione:
Z. Machaty et al., "EFFECTS OF INJECTING CALCIUM-CHLORIDE INTO IN VITRO-MATURED PORCINE OOCYTES", Biology of reproduction, 54(2), 1996, pp. 316-322

Abstract

In vitro-matured porcine oocytes were given injections of 0.1 M CaCl2and after 6 h evaluated for signs of early and late activation events. CaCl2 injection caused cortical granule exocytosis in 75% (3 of 4) of the oocytes tested. It also induced cell cycle resumption as monitored by the histone H1 kinase assay: the phosphorylation rate of histoneH1 decreased to 36.7% of the original value. Treated oocytes completed meiosis, extruded the second polar body, and progressed to first interphase: 79.4% of them formed one or more pronuclei. The elevated intracellular Ca2+ level resulted in activation-related changes in the protein synthetic profile in 90% (9 of 10) of the oocytes. Furthermore, 14.7% (9 of 61) of the treated oocytes developed to the compact morula/early blastocyst stage after a 7-day culture in ligated porcine oviduct, and one blastocyst hatched from the zona pellucida. Control oocytesgiven injections of 0.1 M MgCl2 or carrier medium (10 mM Hepes) did not show the changes mentioned. The results strengthen the idea that Ca2+ is a cell messenger that plays a central part in oocyte activation;it is concluded that elevated intracellular Ca2+ level caused by a single injection of CaCl2 leads to both early and late events of porcineoocyte activation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 04:57:07