Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
NORMAL HUMAN ENDOMETRIAL CELLS IN CULTURE - CHARACTERIZATION AND IMMORTALIZATION OF EPITHELIAL AND STROMAL CELLS BY SV-40 LARGE T-ANTIGEN
Autore:
MERVIEL P; DEGEORGES A; SALATBAROUX J; CALVO F;
Indirizzi:
HOP TENON,SERV GYNECOL OBSTET,4 RUE DE LA CHINE F-75020 PARIS FRANCE HOP ST LOUIS,INST GENET MOLEC,PHARMACOL LAB F-75010 PARIS FRANCE
Titolo Testata:
Biology of the cell
fascicolo: 3, volume: 84, anno: 1995,
pagine: 187 - 193
SICI:
0248-4900(1995)84:3<187:NHECIC>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXTRACELLULAR-MATRIX; VIMENTIN-TYPE; GROWTH; EXPRESSION; FILAMENTS; LINES;
Keywords:
EPITHELIAL ENDOMETRIAL CELLS; IMMORTALIZATION; STROMAL ENDOMETRIAL CELLS; SV 40 LARGE T ANTIGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
P. Merviel et al., "NORMAL HUMAN ENDOMETRIAL CELLS IN CULTURE - CHARACTERIZATION AND IMMORTALIZATION OF EPITHELIAL AND STROMAL CELLS BY SV-40 LARGE T-ANTIGEN", Biology of the cell, 84(3), 1995, pp. 187-193

Abstract

Thirty endometrial biopsies were cultured in order to separate stromal and epithelial cells. Using epidermal growth factor (EGF), cortisol,cholera toxin, insulin with 5% horse serum for epithelial cells or a medium with 20% fetal calf serum for stromal cells, we could specifically enrich endometrial culture in epithelial or stromal cells and culture them for 1 or 2 months. These cultures retained the phenotypic characteristics of epithelial (cytokeratins, mucin HMFG 1) and stromal (vimentin, smooth muscle actin, desmin) lineage by immunostaining analysis. Epithelial and stromal cultures from one individual were respectively immortalized by the SV 40 large T antigen. The immortalized cell lines kept the phenotype of the normal cells from which they derived.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 10:25:51