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Titolo:
CATIONIC PROTEINS INCREASE THE PERMEABILITY OF CULTURED RABBIT TRACHEAL EPITHELIAL-CELLS - MODIFICATION BY HEPARIN AND EXTRACELLULAR CALCIUM
Autore:
UCHIDA DA; IRVIN CG; BALLOWE C; LARSEN G; COTT GR;
Indirizzi:
UNIV UTAH,SCH MED,DEPT PEDIAT,50 N MED DR SALT LAKE CITY UT 84103 NATL JEWISH CTR IMMUNOL & RESP MED,DEPT PEDIAT DENVER CO 80206 UNIV COLORADO,SCH MED,DEPT PEDIAT,SECT PEDIAT PULM MED DENVER CO 00000 NATL JEWISH CTR IMMUNOL & RESP MED,DEPT MED DENVER CO 80206
Titolo Testata:
Experimental lung research
fascicolo: 1, volume: 22, anno: 1996,
pagine: 85 - 99
SICI:
0190-2148(1996)22:1<85:CPITPO>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR BASIC-PROTEIN; AIRWAY HYPERRESPONSIVENESS; BRONCHIAL HYPERREACTIVITY; ELECTRICAL-PROPERTIES; ION-TRANSPORT; MILD ASTHMA; EOSINOPHILS; MONOLAYERS; JUNCTIONS; MUCOSA;
Keywords:
EPITHELIAL FUNCTION; ELECTRICAL RESISTANCE; ASTHMA; CATIONIC PROTEINS; HEPARIN; CALCIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
D.A. Uchida et al., "CATIONIC PROTEINS INCREASE THE PERMEABILITY OF CULTURED RABBIT TRACHEAL EPITHELIAL-CELLS - MODIFICATION BY HEPARIN AND EXTRACELLULAR CALCIUM", Experimental lung research, 22(1), 1996, pp. 85-99

Abstract

Airway inflammation is a consistent finding in asthma, and increased amounts of eosinophil-derived cationic proteins are present in bronchoalveolar lavage fluid from asthmatic subjects. Tracheal instillation of a variety of naturally occurring and synthetic cationic proteins hasbeen shown to induce airway hyperresponsiveness in animal models. Cationic proteins may alter the barrier function of airway epithelium, allowing increased access of agonists to underlying nerves and airway smooth muscle. To examine the effect of cationic proteins on airway epithelial cell function, rabbit tracheal epithelial cells were isolated and cultured on collagen-coated filter membranes. Both apical and basolateral exposure of the cell cultures to poly-L-lysine and poly-L-arginine decreased transepithelial electrical resistance (R(t)) over 60 min. There were no discernable light microscopic changes in the morphology of the cultures at 60 min after poly-L-lysine exposure, but permeability to mannitol was increased compared to controls. Evidence for the critical role of cationic charge included the following observations: (1) Poly-L-aspartate, an anionic polyamino acid, had no significant effect on R(t), and (2) the addition of heparin prior to the addition ofpoly-L-lysine blocked the reduction in R(t). Furthermore, when applied after poly-L-lysine addition, heparin reversed the decrease in R(t) in a time-dependent fashion. Increasing the (Ca2+] in the medium from 1 to 10 mM resulted in significant attenuation of the response to polycation addition. These findings suggest that cationic proteins significantly alter the barrier properties of airway epithelium and that cationic charge is a crucial factor. This alteration is not an ''all or none'' phenomenon, since subsequent addition of heparin resulted in a reversal of the effect. While the precise mechanisms responsible for these observations remain to be elucidated, cationic proteins may be modifying the interaction of extracellular calcium with tight junctions thereby resulting in increased permeability. The barrier function of theepithelium may be perturbed in asthma and a variety of other airway diseases through the presence of cationic proteins derived from inflammatory cells within the airway lumen and/or the subepithelium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 16:10:45