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Titolo:
TOXOPLASMA-GONDII INFECTION INDUCES SPECIFIC NONRESPONSIVENESS IN LYMPHOCYTES BEARING THE V-BETA-5 CHAIN OF THE MOUSE T-CELL RECEPTOR
Autore:
DENKERS EY; CASPAR P; HIENY S; SHER A;
Indirizzi:
CORNELL UNIV,COLL VET MED,DEPT MICROBIOL & IMMUNOL ITHACA NY 14853 NIAID,PARASIT DIS LAB,IMMUNOBIOL SECT BETHESDA MD 20892
Titolo Testata:
The Journal of immunology
fascicolo: 3, volume: 156, anno: 1996,
pagine: 1089 - 1094
SICI:
0022-1767(1996)156:3<1089:TIISNI>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
STAPHYLOCOCCAL ENTEROTOXIN-B; TUMOR-NECROSIS-FACTOR; NATURAL-KILLER-CELLS; INTERFERON-GAMMA; FACTOR-ALPHA; IMMUNE-RESPONSES; IN-VIVO; MICE; SUPERANTIGEN; RESISTANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
E.Y. Denkers et al., "TOXOPLASMA-GONDII INFECTION INDUCES SPECIFIC NONRESPONSIVENESS IN LYMPHOCYTES BEARING THE V-BETA-5 CHAIN OF THE MOUSE T-CELL RECEPTOR", The Journal of immunology, 156(3), 1996, pp. 1089-1094

Abstract

We recently reported a superantigen activity associated with Toxoplasma gondii tachyzoites that in vitro induces preferential expansion of V beta 5(+) T lymphocytes following parasite stimulation of nonimmune cells. In the experiments presented in this work, V beta 5(+) lymphocyte function was examined ex vivo using mice undergoing chronic and acute infection with the avirulent parasite strain ME49 or acutely infected with the attenuated mutant ts-4, Cells bearing the TCR V beta 5 chain were found to be increased by 1.5- to twofold during acute infection, whereas during the chronic phase, modest decreases (similar to 20%)in cells of the latter subset were observed. When splenocytes from chronically infected animals were stimulated in vitro with tachyzoites, the preferential expansion of V beta 5(+) lymphocytes seen using cellsfrom normal mice was not observed. Furthermore, when purified T lymphocytes were cultured with plate-bound V beta 5-specific mAb, we found that in contrast to normal and acutely infected animals, cells from chronically infected and ts-4-vaccinated mice were nonresponsive to TCR-induced stimulation (70 to 90% reduction relative to normal cells). Incontrol experiments, mAb to CD3 and V beta 8 elicited normal responses in the same animals. Similarly, in contrast to normal splenocytes, cells from chronically infected mice failed to produce IFN-gamma in response to anti-V beta 5 mAb. These data indicate that V beta 5(+) tellsare rendered nonresponsive as a result of in vivo encounter with T. gondii, and as such they provide the first demonstration of V beta-specific anergy induced by a protozoan parasite.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 00:00:46