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Titolo:
SENSORY TRANSDUCTION IN VOMERONASAL NEURONS - EVIDENCE FOR G-ALPHA-O,G-ALPHA-I2, AND ADENYLYL-CYCLASE-II AS MAJOR COMPONENTS OF A PHEROMONE SIGNALING CASCADE
Autore:
BERGHARD A; BUCK LB;
Indirizzi:
HARVARD UNIV,SCH MED,HOWARD HUGHES MED INST,DEPT NEUROBIOL,220 LONGWOOD AVE BOSTON MA 02115 HARVARD UNIV,SCH MED,HOWARD HUGHES MED INST,DEPT NEUROBIOL BOSTON MA 02115
Titolo Testata:
The Journal of neuroscience
fascicolo: 3, volume: 16, anno: 1996,
pagine: 909 - 918
SICI:
0270-6474(1996)16:3<909:STIVN->2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
OLFACTORY MARKER PROTEIN; DIFFERENTIAL LOCALIZATION; ODORANT DETECTION; GAMMA-SUBUNITS; RAT-BRAIN; RECEPTOR; EPITHELIUM; SYSTEM; ORGANIZATION; EXPRESSION;
Keywords:
VOMERONASAL; G-PROTEIN; OLFACTORY; SIGNAL TRANSDUCTION; CHEMOSENSORY; ADENYLYL CYCLASE; PHEROMONE; JACOBSONS ORGAN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
A. Berghard e L.B. Buck, "SENSORY TRANSDUCTION IN VOMERONASAL NEURONS - EVIDENCE FOR G-ALPHA-O,G-ALPHA-I2, AND ADENYLYL-CYCLASE-II AS MAJOR COMPONENTS OF A PHEROMONE SIGNALING CASCADE", The Journal of neuroscience, 16(3), 1996, pp. 909-918

Abstract

The mammalian vomeronasal organ (VNO) is an accessory olfactory structure implicated in the sensing of pheromones. Although virtually nothing is known about sensory transduction in the mammalian VNO, recent findings have raised the possibility that it proceeds via a G-protein-coupled mechanism and involves a cyclic nucleotide-gated ion channel as in the nasal olfactory epithelium. To investigate this possibility, wecloned G-protein alpha subunits, adenylyl cyclases, and guanylyl cyclases that are expressed in the VNO and examined their patterns of expression. Of seven G alpha subunits identified as being expressed in theVNO, we found that mRNAs encoding only two, G alpha o and G alpha i2,are highly expressed in VNO neurons. Moreover, G alpha o and G alpha i2 are highly expressed by separate subsets of neurons that are located in different regions of the VNO neuroepithelium. Immunohistochemicalstudies show that both G alpha o and G alpha i2 are enriched in VNO microvilli, suggesting that G-proteins containing both of these alpha subunits may be involved in VNO sensory transduction. Of the adenylyl and guanylyl cyclases that we cloned, we found that only one, adenylyl cyclase type II, is highly expressed in VNO neurons; furthermore, it is expressed by both G alpha o(+) and G alpha i2(+) subsets. Our findings suggest that spatially segregated subsets of VNO neurons may use different, but related, sensory transduction pathways in which G-proteins and an adenylyl cyclase play major roles.

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Documento generato il 21/09/20 alle ore 18:55:39