Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
CD14(-COLONY-STIMULATING FACTOR (G-CSF)-MOBILIZED PERIPHERAL-BLOOD MONONUCLEAR-CELLS INDUCE SECRETION OF INTERLEUKIN-6 AND G-CSF BY MARROW STROMA() CELLS IN GRANULOCYTE)
Autore:
MIELCAREK M; ROECKLEIN BA; TOROKSTORB B;
Indirizzi:
FRED HUTCHINSON CANC RES CTR,DIV CLIN RES,PROGRAM TRANSPLANTAT BIOL,1124 COLUMBIA ST,M-318 SEATTLE WA 98104 UNIV WASHINGTON,DEPT MED SEATTLE WA 98195
Titolo Testata:
Blood
fascicolo: 2, volume: 87, anno: 1996,
pagine: 574 - 580
SICI:
0006-4971(1996)87:2<574:CF(PM>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-DOSE CHEMOTHERAPY; AUTOLOGOUS BONE-MARROW; PLATELET RECOVERY; TRANSPLANTATION; PRIMATES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
M. Mielcarek et al., "CD14(-COLONY-STIMULATING FACTOR (G-CSF)-MOBILIZED PERIPHERAL-BLOOD MONONUCLEAR-CELLS INDUCE SECRETION OF INTERLEUKIN-6 AND G-CSF BY MARROW STROMA() CELLS IN GRANULOCYTE)", Blood, 87(2), 1996, pp. 574-580

Abstract

The ability of granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood mononuclear cells (G-PBMCs) to induce secretion of cytokines in primary longterm marrow cultures (LTC) or in the human marrow stromal cell line HS23 was compared with that of marrow mononuclear cells. Equal numbers of G-PBMCs or marrow mononuclear cells were added to stromal cultures, supernatants were harvested at day 4 and levels of interleukin-1 alpha (IL-1 alpha), IL-1 beta, IL-2, IL-6, G-CSF, and tumor necrosis factor alpha (TNF alpha) were determined. G-PBMCs induced 21.4-fold higher levels of IL-6 and 12.5-fold higher levels of G-CSF in LTC cocultures compared with marrow mononuclear cells and induced 20.6-fold more IL-6 and 6.3-fold more G-CSF when added to HS23 cells. Experiments using sorted populations of CD20(+), CD3(+), and CD14(+) cells showed that CD14(+) cells within G-PBMCs were responsible for triggering the production of IL-6 and G-CSF. The effect did not require cell-cell contact and was inhibited when neutralizing antibodies to lL-1 alpha and IL-1 beta were used in combination. In these experiments, the greater stimulating ability of G-PBMCs is most likely attributable to the greater number of CD14(+) cells in G-PBMCs (26.1% +/- 2.3%) compared with marrow (2.5% +/- 0.8%), because equal numbers of CD14(+) cells sorted from marrow and G-PBMCs showed comparable ability to induce IL-6 and G-CSF when placed directly on stromal cells. (C) 1996 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 09:00:22