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Titolo:
RELATIONSHIPS AMONG MORPHOLOGICAL STATUS, STEROID-HORMONES, AND POSTTHAWING VIABILITY OF FROZEN SPERMATOZOA OF MALE MINKE WHALES (BALAENOPTERA-ACUTOROSTRATA)
Autore:
FUKUI Y; MOGOE T; JUNG YG; TERAWAKI Y; MIYAMOTO A; ISHIKAWA H; FUJISE Y; OHSUMI S;
Indirizzi:
OBIHIRO UNIV AGR & VET MED,LAB ANIM GENET & REPROD OBIHIRO HOKKAIDO 080 JAPAN INST CETACEAN RES,CHUO KU TOKYO 104 JAPAN
Titolo Testata:
Marine mammal science
fascicolo: 1, volume: 12, anno: 1996,
pagine: 28 - 37
SICI:
0824-0469(1996)12:1<28:RAMSSA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
TURSIOPS-TRUNCATUS; SERUM;
Keywords:
SPERMATOZOA; CRYOPRESERVATION; STEROID HORMONES; MINKE WHALE; BALAENOPTERA ACUTOROSTRATA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
Y. Fukui et al., "RELATIONSHIPS AMONG MORPHOLOGICAL STATUS, STEROID-HORMONES, AND POSTTHAWING VIABILITY OF FROZEN SPERMATOZOA OF MALE MINKE WHALES (BALAENOPTERA-ACUTOROSTRATA)", Marine mammal science, 12(1), 1996, pp. 28-37

Abstract

Spermatozoa from 21 mature minke whales (Balaenoptera acutorostrata) taken in the Antarctic Ocean for Japanese research were recovered fromvasa deferentia, diluted 1:9 in a Tris-based diluent, and frozen at -80 degrees C on board the vessel. After a period ranging from 45 to 125 d, the samples were transferred to liquid nitrogen and transported to the laboratory. After thawing at 37 degrees C, the motility (percentage of motile spermatozoa), vitality (proportion of live spermatozoa),and sperm concentration were determined for each sample. These valueswere tested for correlations with morphological measurements (body size, body weight, testis weight) and serum concentrations of progesterone (P-4), estradiol-17 beta (E(2)), and testosterone (T). Ten of 21 samples had motile spermatozoa (2%-40%). Although no motile spermatozoa were observed in 11 samples, all sperm samples were examined by eosin-nigrosin staining and showed vitality levels of 3%-44%. It was found that the motility (r = 0.54) and vitality (r = 0.53) of the spermatozoawere significantly (P < 0.01) correlated with the E(2) levels (8.50 +/- 1.80 pg/ml). Serum T levels (0.07 +/- 0.02 ng/ml) were significantly correlated with the E(2) levels (r = 0.58, P < 0.01), but sperm concentrations were not correlated with either E(2) Or T levels. The present study demonstrates that spermatozoa of minke whales can be successfully cryopreserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 10:15:52