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Titolo:
CLONING AND CHARACTERIZATION OF A CONSERVED REGION OF HUMAN AND RHESUS MACAQUE PNEUMOCYSTIS-CARINII GPA
Autore:
WRIGHT TW; GIGLIOTTI F; HAIDARIS CG; SIMPSONHAIDARIS PJ;
Indirizzi:
UNIV ROCHESTER,SCH MED & DENT,DEPT MED,HEMATOL UNIT,POB 610,601 ELMWOOD AVE ROCHESTER NY 14642 UNIV ROCHESTER,SCH MED & DENT,DEPT MED,HEMATOL UNIT ROCHESTER NY 14642 UNIV ROCHESTER,SCH MED & DENT,DEPT MICROBIOL & IMMUNOL ROCHESTER NY 14642 UNIV ROCHESTER,SCH MED & DENT,DEPT PEDIAT ROCHESTER NY 14642 UNIV ROCHESTER,SCH MED & DENT,DEPT DENT RES ROCHESTER NY 14642 UNIV ROCHESTER,SCH MED & DENT,DEPT PATHOL & LAB MED ROCHESTER NY 14642
Titolo Testata:
Gene
fascicolo: 1-2, volume: 167, anno: 1995,
pagine: 185 - 189
SICI:
0378-1119(1995)167:1-2<185:CACOAC>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAJOR SURFACE GLYCOPROTEIN; RAT; PNEUMONIA; KARYOTYPES; ANTIGENS; FERRET; GENES;
Keywords:
AIDS; GENOTYPE; IMMUNOSUPPRESSED; OPPORTUNISTIC PATHOGEN; PHENOTYPE; PCR; PHYLOGENY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
T.W. Wright et al., "CLONING AND CHARACTERIZATION OF A CONSERVED REGION OF HUMAN AND RHESUS MACAQUE PNEUMOCYSTIS-CARINII GPA", Gene, 167(1-2), 1995, pp. 185-189

Abstract

Although the genes encoding Pneumocystis carinii (Pc) glycoprotein A (gpA) display a high degree of host species-specific genotypic diversity, the Pc gpA derived from different host species share defined regions of significant homology in their primary amino acid (aa) structure. Using two degenerate oligodeoxyribonucleotide (oligo) primers corresponding to a conserved Cys region (Cys-primers) of the ferret (F), rat (R) and mouse (M) PcgpA, a 306-bp portion of the human (H) PcgpA was amplified from only one of three known HPc-infected lung samples using PCR. The deduced aa sequence of the HPc PCR product was 72% similar tothe corresponding region of a published HPc gpA aa sequence, Because the conserved Cys-primers amplified only one of three samples of HPcgpA, a primer-pair was designed from sequences internal to the Cys-primer sequences of the HPcgpA PCR product (hPc). The hPc primers amplifiedthe expected 254-bp product from each of the three HPc-infected lung DNA samples, suggesting that the Cys-primers may have either amplifieda HPcgpA present in fewer copies in the genome of HPc or, alternatively, amplified a gene from an uncommon strain of Pc encoding an isoformvariant of gpA not present in the other human isolates analyzed in this report. Restriction analysis of the amplified products demonstratedheterogeneity in the internal sequence, confirming that more than onegpA exists in HPc as well. To determine the relationship of HPcgpA tothe gpA of Pc from another primate, the hPc primers were used successfully to amplify a 261-bp product from Pc-infected Rhesus macaque (Rm)lung genomic DNA, These results are consistent with our earlier findings that closely related host species are infected with Pc organisms encoding similar gpA, suggesting that the evolutionary divergence of Pcfollowed that of the mammalian host species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 00:46:18