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Titolo:
ANALYSIS OF DOUBLE-STRANDED POLYMERASE CHAIN-REACTION PRODUCTS FROM THE BACILLUS-CEREUS GROUP BY ELECTROSPRAY-IONIZATION FOURIER-TRANSFORM ION-CYCLOTRON RESONANCE MASS-SPECTROMETRY
Autore:
WUNSCHEL DS; FOX KF; FOX A; BRUCE JE; MUDDIMAN DC; SMITH RD;
Indirizzi:
UNIV S CAROLINA,SCH MED,DEPT MICROBIOL & IMMUNOL COLUMBIA SC 29209 UNIV S CAROLINA,SCH MED,DEPT MICROBIOL & IMMUNOL COLUMBIA SC 29209 PACIFIC NW NATL LAB,ENVIRONM MOLEC SCI LAB RICHLAND WA 99352
Titolo Testata:
Rapid communications in mass spectrometry
fascicolo: 1, volume: 10, anno: 1996,
pagine: 29 - 35
SICI:
0951-4198(1996)10:1<29:AODPCP>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
16S RIBOSOMAL-RNA; ANTHRACIS; ACID; THURINGIENSIS; BACTERIA; PROTEINS; MATRIX;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
D.S. Wunschel et al., "ANALYSIS OF DOUBLE-STRANDED POLYMERASE CHAIN-REACTION PRODUCTS FROM THE BACILLUS-CEREUS GROUP BY ELECTROSPRAY-IONIZATION FOURIER-TRANSFORM ION-CYCLOTRON RESONANCE MASS-SPECTROMETRY", Rapid communications in mass spectrometry, 10(1), 1996, pp. 29-35

Abstract

The analysis of polymerase chain reaction (PCR) products by electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR) has been achieved. Specifically, a 105 base-pair nucleotide portion of the ribosomal spacer region was amplified in two members of the B. cereus group (i.e. B. thuringiensis and B. cereus) using PCR. These amplified regions were then analyzed by gel electrophoresis and ESI-FTICR. Based on the predicted sequence of the PCR products for each organism, the mass measurement using ESI-FTICR matched the theoretical mass within experimental error and was consistent with gel electrophoresis results. In contrast, for the typical several hour time-scale of the gel electrophoresis experiment, the mass spectrometric analysis was completed in a matter of minutes. To our knowledge, this constitutes the first report demonstrating the ionization and detectionof a double-stranded PCR product by ESI-MS. This preliminary result indicates the potential use of ESI-MS to analyze PCR products on a rapid time-scale, with potential for medical and taxonomic applications.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 04:48:22