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Titolo: ANTISPERM ANTIBODY-BINDING TO HUMAN SPERM INHIBITS CAPACITATION INDUCED CHANGES IN THE LEVELS OF PLASMA-MEMBRANE STEROLS
Autore: BENOFF S; COOPER GW; HURLEY I; MANDEL FS; ROSENFELD DL;
- Indirizzi:
- N SHORE UNIV HOSP,BOAS MARKS BIOMED SCI RES CTR,DIV HUMAN REPROD,MOLEC BIOL LAB MANHASSET NY 11030 KEMRON ENVIRONM SERV,HUNTINGTON STN NEW YORK NY 00000 N SHORE UNIV HOSP,DEPT RES MANHASSET NY 11030 N SHORE UNIV HOSP,DEPT OBSTET & GYNECOL MANHASSET NY 11030 CORNELL UNIV,MED CTR,COLL MED,DEPT OBSTET & GYNECOL NEW YORK NY 10021 CORNELL UNIV,MED CTR,COLL MED,DEPT PUBL HLTH NEW YORK NY 10021
- Titolo Testata:
- American journal of reproductive immunology [1989]
fascicolo: 2-3,
volume: 30,
anno: 1993,
pagine: 113 - 130
- SICI:
- 1046-7408(1993)30:2-3<113:AATHSI>2.0.ZU;2-7
- Fonte:
- ISI
- Lingua:
- ENG
- Soggetto:
- HUMAN FOLLICULAR-FLUID; FREE HAMSTER OVA; ACROSOME REACTION; HUMAN-SPERMATOZOA; ZONA-PELLUCIDA; PROGESTERONE-RECEPTOR; INVITRO CAPACITATION; MONOCLONAL-ANTIBODY; CHOLESTEROL EFFLUX; INFERTILE PATIENTS;
- Keywords:
- ANTISPERM ANTIBODY; MEMBRANE CHOLESTEROL; MANNOSE LIGAND RECEPTORS; SPERM SURFACE;
- Tipo documento:
- Article
- Natura:
- Periodico
- Settore Disciplinare:
- Science Citation Index Expanded
- Science Citation Index Expanded
- Citazioni:
- 80
- Recensione:
- Indirizzi per estratti:
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- Citazione:
- S. Benoff et al., "ANTISPERM ANTIBODY-BINDING TO HUMAN SPERM INHIBITS CAPACITATION INDUCED CHANGES IN THE LEVELS OF PLASMA-MEMBRANE STEROLS", American journal of reproductive immunology [1989], 30(2-3), 1993, pp. 113-130
Abstract
PROBLEM: Human spermatozoa express mannose ligand receptors (MLRs) over the entire head when incubated under conditions that promote loss of free cholesterol. Binding of IgA, IgG, and/or IgM head-directed anti-sperm antibodies (ASAs) to freshly isolated sperm blocks MLR expression in a dose dependent manner. METHODS: Comparison of Western blots ofimmunoprecipated glycoproteins from fresh sperm exposed to ASAs from women with those of sperm membrane proteins isolated from capacitated sperm probed with a goat anti-human macrophage MLR antiserum showed that ASAs do not react with human sperm MLRs of 48 and 61 kd apparent molecular weight. RESULTS: The direct effect of ASA binding was to increase membrane free cholesterol content which remained greater than 0.005 mumol/10(9) sperm after 18 h incubations, whereas the sterol contentof controls decreased to less than 0.001 mumol/10(9) sperm. Cholesterol addition to sperm inhibited MLR expression in a manner analogous toASA binding, while increasing temperatures up to the crystalline/liquid-crystalline phase transition for sperm membranes; less than 45-degrees-C failed to promote the appearance of MLRs on the surface of freshsperm. CONCLUSIONS: We conclude that ASA effects on membrane cholesterol content prevent the membrane fluidity changes needed for MLR expression.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 04:28:22