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Titolo:
THE PINEAL NEUROHORMONE MELATONIN PREVENTS IN-VIVO AND IN-VITRO APOPTOSIS IN THYMOCYTES
Autore:
SAINT RM; MAYO JC; URIA H; KOTLER M; ANTOLIN I; RODRIGUEZ C; MENENDEZPELAEZ A;
Indirizzi:
UNIV OVIEDO,FAC MED,DEPT MORFOL & BIOL CELULAR,JULIAN CLAVERIA S-N E-33006 OVIEDO SPAIN UNIV OVIEDO,FAC MED,DEPT MORFOL & BIOL CELULAR E-33006 OVIEDO SPAIN
Titolo Testata:
Journal of pineal research
fascicolo: 4, volume: 19, anno: 1995,
pagine: 178 - 188
SICI:
0742-3098(1995)19:4<178:TPNMPI>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; GROWTH-FACTOR DEPRIVATION; CENTRAL-NERVOUS-SYSTEM; DNA FRAGMENTATION; RAT THYMUS; BCL-2; EXPRESSION; RECEPTOR; BINDING; NEURONS;
Keywords:
MELATONIN; APOPTOSIS; THYMUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
68
Recensione:
Indirizzi per estratti:
Citazione:
R.M. Saint et al., "THE PINEAL NEUROHORMONE MELATONIN PREVENTS IN-VIVO AND IN-VITRO APOPTOSIS IN THYMOCYTES", Journal of pineal research, 19(4), 1995, pp. 178-188

Abstract

Recently, melatonin was found to be the most potent physiological free radical scavenger known to date. In this work, we attempted to define the role this neurohormone plays in the regulation of apoptosis, since the effect of bcl-2, the main gene implicated in its inhibition, acts via an antioxidant mechanism. We investigated the role of melatoninin cell death of thymus, a well known model for the study of apoptosis. Two sets of experiments were carried out: in vivo experiments, performed with Wistar rats, and in vitro experiments, performed with primary cultures of young Wistar rat thymocytes treated with glucocorticoids in order to induce apoptosis. Morphometrical studies in semithin sections of thymus and analysis of DNA fragmentation by,eel electrophoresis show that physiological apoptosis occurring in thymus of 65 days old rats, is prevented by the daily administration of melatonin beginning when the rats were 25 days old. Also, we found that at a concentration of 10(-7) M, melatonin decreases by 35% the percentage of apoptoticcells induced by glucocorticoids in cultured thymocytes of 25 day oldrats. 10(-9) M melatonin decreases cell death by 20%. Finally, melatonin at 10(-11) M did not have any effect. Several hypothesis are discussed to explain this effect: direct interaction of melatonin with glucocorticoid receptors in the thymus; induction of interleukin-4 release; direct genomic action modulating the expression of apoptosis-inhibiting genes; an effect on nitric oxide synthase; and finally, the antioxidant action of melatonin. Since apoptosis is a possible mechanism involved in neuronal death shown in several neurodegenerative diseases such as Parkinson or Alzheimer's diseases, investigative efforts should be directed to the possible role of melatonin in inhibiting cell deathin tissues other that the thymus. Melatonin might be a potent therapeutic agent in some of these conditions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 17:59:40