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Titolo:
XKLP2, A NOVEL XENOPUS CENTROSOMAL KINESIN-LIKE PROTEIN REQUIRED FOR CENTROSOME SEPARATION DURING MITOSIS
Autore:
BOLETI H; KARSENTI E; VERNOS I;
Indirizzi:
EUROPEAN MOLEC BIOL LAB,CELL BIOL PROGRAMME,MEYERHOFSTR 1 D-69117 HEIDELBERG GERMANY EUROPEAN MOLEC BIOL LAB,CELL BIOPHYS PROGRAMME D-69117 HEIDELBERG GERMANY
Titolo Testata:
Cell
fascicolo: 1, volume: 84, anno: 1996,
pagine: 49 - 59
SICI:
0092-8674(1996)84:1<49:XANXCK>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPINDLE FORMATION; EXTRACTS; SEQUENCES; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
H. Boleti et al., "XKLP2, A NOVEL XENOPUS CENTROSOMAL KINESIN-LIKE PROTEIN REQUIRED FOR CENTROSOME SEPARATION DURING MITOSIS", Cell, 84(1), 1996, pp. 49-59

Abstract

We describe a novel Xenopus plus end-directed kinesin-like protein (KLP), Xklp2, localized on centrosomes throughout the cell cycle and on spindle pole microtubules during metaphase. Using mitotic spindles assembled in Xenopus egg extracts and different recombinant GST-Xklp2 mutants, we show that this motor is targeted to spindle poles through itsC-terminal domain. Xklp2-truncated polypeptides lacking the motor domain block centrosome separation and disrupt preassembled metaphase spindles. Antibodies directed against the tail of Xklp2 have a similar effect. These results show that Xklp2 protein is required for centrosomeseparation and maintenance of spindle bipolarity. This study is an example of the application of the dominant negative mutant effect on spindle assembly in Xenopus egg extracts, demonstrating the usefulness ofthis approach in probing the function of proteins in this system.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 00:46:29