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Titolo:
INHIBITION OF TETRAHYDROBIOPTERIN SYNTHESIS REDUCES NITRIC-OXIDE PRODUCTION BY ISOLATED GLOMERULI IN IMMUNE-COMPLEX GLOMERULONEPHRITIS
Autore:
BUNE AJ; COOK HT;
Indirizzi:
UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,ST MARYS HOSP,SCH MED,DEPT HISTOPATHOL LONDON W2 1PG ENGLAND UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,ST MARYS HOSP,SCH MED,DEPT HISTOPATHOL LONDON W2 1PG ENGLAND
Titolo Testata:
Experimental nephrology
fascicolo: 1, volume: 4, anno: 1996,
pagine: 43 - 47
SICI:
1018-7782(1996)4:1<43:IOTSRN>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASCULAR SMOOTH-MUSCLE; L-ARGININE; MURINE MACROPHAGES; ENDOTHELIAL-CELLS; SYNTHASE; INJURY; RAT; BIOSYNTHESIS; GENERATION; EXPRESSION;
Keywords:
NEPHROTOXIC NEPHRITIS, ACCELERATED; TETRAHYDROBIOPTERIN; GLOMERULONEPHRITIS; NITRIC OXIDE; LIPOPOLYSACCHARIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
A.J. Bune e H.T. Cook, "INHIBITION OF TETRAHYDROBIOPTERIN SYNTHESIS REDUCES NITRIC-OXIDE PRODUCTION BY ISOLATED GLOMERULI IN IMMUNE-COMPLEX GLOMERULONEPHRITIS", Experimental nephrology, 4(1), 1996, pp. 43-47

Abstract

We studied the effect of modulators of tetrahydrobiopterin (BH4) synthesis on the production of nitrite by glomeruli from rats with immune complex-mediated glomerular inflammation. Glomeruli were isolated 24 hafter induction of accelerated nephrotoxic nephritis and cultured at 2,000/ml for 38 h. Lipopolysaccharide dose dependently increased ex vivo nitrite production. 2,4-Diamino-6-hydroxypyrimidine (DAHP), an inhibitor of the de novo synthetic pathway of BH4, inhibited nitrite production by up to 50% and this effect was reversed by sepiapterin, a substrate for the salvage pathway of BH4 synthesis. Sepiapterin alone increased nitrite generation indicating that BH4 is rate limiting. Inhibition of both pathways with DAHP and methotrexate led to 80% inhibition of nitrite. Pharmacological inhibition of BH4 synthesis may be a useful mechanism to reduce nitric oxide synthesis in immune complex inflammation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 19:51:40