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Titolo:
STRUCTURE OF GLYCOPEPTIDES ISOLATED FROM BOVINE-MILK COMPONENT PP3
Autore:
GIRARDET JM; CODDEVILLE B; PLANCKE Y; STRECKER G; CAMPAGNA S; SPIK G; LINDEN G;
Indirizzi:
UNIV NANCY 1,FAC SCI,LAB BIOSCI ALIMENT,INRA,B P 239 F-54506 VANDOEUVRE NANCY FRANCE UNIV SCI & TECHNOL,UNITE MIXTE RECH,CNRS NO 111,CHIM BIOL LAB LILLE FRANCE
Titolo Testata:
European journal of biochemistry
fascicolo: 3, volume: 234, anno: 1995,
pagine: 939 - 946
SICI:
0014-2956(1995)234:3<939:SOGIFB>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAS-LIQUID-CHROMATOGRAPHY; FAT GLOBULE-MEMBRANE; MAGNETIC-RESONANCE SPECTROSCOPY; PROTEOSE-PEPTONE FRACTION; METHYL GLYCOSIDES; GLYCOPROTEIN GLYCANS; MASS-SPECTROMETRY; SUGAR CHAINS; GLYCOSYLATION; LUTROPIN;
Keywords:
BOVINE MILK; PROTEOSE PEPTONE; COMPONENT PP3; GLYCOPROTEIN; GLYCAN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Girardet et al., "STRUCTURE OF GLYCOPEPTIDES ISOLATED FROM BOVINE-MILK COMPONENT PP3", European journal of biochemistry, 234(3), 1995, pp. 939-946

Abstract

The heat-stable acid-soluble phosphoglycoprotein component PP3 was isolated from the bovine milk proteose peptone fraction by concanavalin A affinity chromatography. Glycopeptides were released by pronase digestion of the milk component PP3 and were subsequently separated by high-pH anion-exchange chromatography on CarboPac PA-1. The primary structures of the glycan and peptide moieties of eight N-glycopeptides havebeen established by combining methylation analysis, mass spectrometry, 400-MHz H-1-NMR spectroscopy, and peptide sequence analysis. All theanalyzed fractions contained biantennary N-acetyllactosamine-type carbohydrate chains, some of them with a GaInAc(beta 1-4)GlcNAc or a NeuAc(alpha 2-6)GalNAc(beta 1-4)GlcNAc group. This particular sequence didor did not replace the Gal(beta 1-4)GlcNAc group usually found in most N-linked glycans. Moreover, the sialylated Gal and GalNAc residues were only found on the Man(alpha 1-3) antenna.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 04:08:34