Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
THE THROMBOXANE MIMETIC, U-46619, INDUCES THE AGGREGATION OF FISH THROMBOCYTES
Autore:
HILL DJ; ROWLEY AF;
Indirizzi:
UNIV COLL SWANSEA,SCH BIOL SCI,SINGLETON PK SWANSEA SA2 8PP W GLAM WALES UNIV COLL SWANSEA,SCH BIOL SCI SWANSEA SA2 8PP W GLAM WALES
Titolo Testata:
British Journal of Haematology
fascicolo: 1, volume: 92, anno: 1996,
pagine: 200 - 211
SICI:
0007-1048(1996)92:1<200:TTMUIT>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-PLATELET-AGGREGATION; WHOLE-BLOOD; ADHESION;
Keywords:
THROMBOCYTES; FISH; HEMOSTASIS; U-46619; FIBRINOGEN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
D.J. Hill e A.F. Rowley, "THE THROMBOXANE MIMETIC, U-46619, INDUCES THE AGGREGATION OF FISH THROMBOCYTES", British Journal of Haematology, 92(1), 1996, pp. 200-211

Abstract

Fish blood contains nucleated cells termed thrombocytes which are thought to be functionally analogous to mammalian platelets. The present study was undertaken to assess the aggregatory response of peripheral blood thrombocytes from the rainbow trout, Oncorhynchus mykiss. Incubation of Percoll density gradient purified thrombocytes with the thromboxane A(2) mimetic, U-46619 (0.03-10 mu M), alone or in the presence of human or trout fibrinogen elicited a dose-dependent. aggregatory response. A greater amount of aggregation was observed in the presence offish, rather than human, fibrinogen (e.g. c 55% compared with 15% maximal aggregation respectively), This response was inhibited by pre-incubation of thrombocytes with the specific thromboxane A(2) receptor antagonist, GR32191 (0.01-10 mu M) with an IC50 value of 5.7 mu M. Ultrastructural and aggregometry studies of thrombocyte aggregation at various time periods (1-12 min) after incubation with U-46619 (0.5 mu M) revealed clear differences between the amount and dynamics of thrombocyte clumping in the presence of human, compared with trout, fibrinogen (final concentration 400 mu g ml(-1)). Thrombocytes rapidly underwent shape change and aggregation after only 1 min incubation without the initial involvement of any other cell types. The maximum degree of aggregation was achieved after 4-8 min with larger aggregates formed in the presence of trout compared than human fibrinogen, Ultrastructurally,the thrombocytes in these aggregates displayed a number of changes compared with nonstimulated cells, including increased pseudopodial activity, a more pronounced canalicular system and condensation of the nuclear heterochromatin., After 12 min incubation the clumps of thrombocytes showed progressive disaggregatory behaviour, with some cells reverting back to their normal in vivo appearance. Neutrophilic granulocytes, present as contaminants in the thrombocyte suspensions, were occasionally found attached to the thrombocyte aggregates. perhaps suggesting a specific interaction similar to that occurring in mammals. Finally, and also of some significance, was the finding that trout thrombocytes exhibit phagocytic activity in vitro towards cell debris and contaminating bacteria, indicating that this cell type may possess both haemostatic and immunological capacities.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 19:28:55