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Titolo:
PROTEIN VARIABILITY IN CEREAL CYST NEMATODES FROM DIFFERENT GEOGRAPHIC REGIONS ASSESSED BY 2-DIMENSIONAL GEL-ELECTROPHORESIS
Autore:
BOSSIS M; RIVOAL R;
Indirizzi:
INRA,LAB RECH CHAIRE ZOOL,CTR RECH RENNES,DOMAINE MOTTE VICONTE,BP 29F-35650 LE RHEU FRANCE
Titolo Testata:
Fundamental and applied nematology
fascicolo: 1, volume: 19, anno: 1996,
pagine: 25 - 34
SICI:
1164-5571(1996)19:1<25:PVICCN>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLOBODERA-ROSTOCHIENSIS; PATTERNS; PATHOTYPES;
Keywords:
CEREAL CYST NEMATODES; HETERODERA AVENAE; PATHOTYPES; 2-DIMENSIONAL ELECTROPHORESIS; PROTEIN PATTERNS; IMAGE COMPUTING ANALYSIS; GENETIC DISTANCES; DENDROGRAMS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
M. Bossis e R. Rivoal, "PROTEIN VARIABILITY IN CEREAL CYST NEMATODES FROM DIFFERENT GEOGRAPHIC REGIONS ASSESSED BY 2-DIMENSIONAL GEL-ELECTROPHORESIS", Fundamental and applied nematology, 19(1), 1996, pp. 25-34

Abstract

The protein variability of twelve populations of cereal cyst nematodes, originating from seven countries (four continents), was studied on three samples of white females per population, reared in controlled conditions on the same wheat, Triticum aestivum cv. Arminda. The pathogenic characteristics of each isolate were obtained from the literature and/or complementary host tests. Three electrophoreses were run for each protein sample with light modifications in migration (O'Farrell, 1975) and staining (Oakley et al., 1980) techniques. 320 polypeptide spots were identified on all the populations tested. The protein patternswere compared by an image computing analysis with a Var station (4000.60), a scanner (Eikonix, Kodak), and a Kepler software combination. Different levels of protein acceptance were applied, based on their volume and/or amplitude range. The similarity indices (F) and genetic distances (D = 1-F) were calculated from identical spots. Dendrograms were built following the UPGMA method. Although some variability was observed between populations, clear genetic dissimilarities separate H. avenae sensu stricto from Gotland strain isolates. In the H. avenae sensu stricto group, two French populations and one isolate from South Australia were very similar. Specific proteins common to both French Ha12and Ha12/Fr2 pathotypes were characterized. The use of image computing analysis to compare protein patterns is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 13:36:49