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Titolo:
ANALYSIS OF NICOTINIC ACETYLCHOLINE-RECEPTOR SUBUNITS IN THE COCHLEA OF THE MOUSE
Autore:
DRESCHER DG; KHAN KM; GREEN GE; MORLEY BJ; BEISEL KW; KAUL H; GORDON D; GUPTA AK; DRESCHER MJ; BARRETTO RL;
Indirizzi:
WAYNE STATE UNIV,SCH MED,DEPT OTOLARYNGOL,LAB BIOOTOL,540 E CANFIELD AVE DETROIT MI 48201 WAYNE STATE UNIV,SCH MED,DEPT BIOCHEM DETROIT MI 48201 AGA KHAN UNIV,DEPT ANAT KARACHI 74800 PAKISTAN BOYS TOWN NATL RES HOSP OMAHA NE 68131
Titolo Testata:
Comparative biochemistry and physiology. Part C, Pharmacology toxicology & endocrinology
fascicolo: 3, volume: 112, anno: 1995,
pagine: 267 - 273
SICI:
1367-8280(1995)112:3<267:AONASI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; GENE FAMILY; FUNCTIONAL DIVERSITY; KINETIC-PROPERTIES; BETA-SUBUNIT; CHOLINERGIC RECEPTOR; XENOPUS OOCYTES; ALPHA-SUBUNIT; GAMMA-SUBUNIT; CDNA CLONES;
Keywords:
ACETYLCHOLINE; COCHLEA; NICOTINIC RECEPTORS; NUCLEOTIDE SEQUENCING; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
D.G. Drescher et al., "ANALYSIS OF NICOTINIC ACETYLCHOLINE-RECEPTOR SUBUNITS IN THE COCHLEA OF THE MOUSE", Comparative biochemistry and physiology. Part C, Pharmacology toxicology & endocrinology, 112(3), 1995, pp. 267-273

Abstract

The present study was designed to catalogue and compare nicotinic receptor subunit messages in the mammalian cochlea, Fourteen nicotinic acetylcholine receptor subunit messages were examined by polymerase chain reaction (PCR) analysis and nucleotide sequencing, Total RNA was extracted from the auditory organs of 14- to 18-day-old CBA(J) mice, and mRNA was purified using oligo-dT cellulose. After reverse transcription, resulting cDNA was amplified by PCR with the use of primers specific for the nucleotide sequences representing the following nicotinic receptor subunits: muscle types alpha 1, beta 1, gamma, delta and epsilon neuronal types alpha 2,3,4,5,6,7 and beta 2,3,4. cDNA from cochlear tissue corresponding to the muscle-type receptor subunit pi and to neuronal-type receptor subunits alpha 2,4,5,6 and beta 2,3 was amplified,whereas cDNA for muscle types alpha 1, gamma, delta, and epsilon and neuronal types alpha 3,7 and beta 4 was not. All PCR products were homologous in nucleotide sequence to the corresponding reference cDNAs from which the primers were designed, The current results indicate that nicotinic acetylcholine receptor (nAChR) subunits that are similar or identical to the stated muscle and neuronal types are expressed in themurine cochlea. The presence of messages corresponding to the muscle type pi and neuronal-type nAChR subunits may be correlated with the atypical cholinergic response of cochlear hair cells to agonists and antagonists.

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Documento generato il 15/07/20 alle ore 05:34:32