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Titolo:
IDENTIFICATION OF AF-6 AND CANOE AS PUTATIVE TARGETS FOR RAS
Autore:
KURIYAMA M; HARADA N; KURODA S; YAMAMOTO T; NAKAFUKU M; IWAMATSU A; YAMAMOTO D; PRASAD R; CROCE C; CANAANI E; KAIBUCHI K;
Indirizzi:
NARA INST SCI & TECHNOL,DIV SIGNAL TRANSDUCT IKOMA 63001 JAPAN NARA INST SCI & TECHNOL,DIV SIGNAL TRANSDUCT IKOMA 63001 JAPAN KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL YOKOHAMA KANAGAWA 236 JAPAN MITSUBISHI KASEI INST LIFE SCI MACHIDA 194 TOKYO JAPAN ERATO YAMAMOTO BEHAV GENES PROJECT MACHIDA TOKYO 194 JAPAN THOMAS JEFFERSON UNIV PHILADELPHIA PA 19107 WEIZMANN INST SCI IL-76100 REHOVOT ISRAEL
Titolo Testata:
The Journal of biological chemistry
fascicolo: 2, volume: 271, anno: 1996,
pagine: 607 - 610
SICI:
0021-9258(1996)271:2<607:IOAACA>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-SUPPRESSOR PROTEIN; SIGNAL-TRANSDUCTION; SEPTATE JUNCTIONS; CLONING; HOMOLOG; GENE; ZO-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
M. Kuriyama et al., "IDENTIFICATION OF AF-6 AND CANOE AS PUTATIVE TARGETS FOR RAS", The Journal of biological chemistry, 271(2), 1996, pp. 607-610

Abstract

Ras (Ha-Ras, Ki-Ras, N-Ras) is implicated in the regulation of various cell functions such as gene expression and cell proliferation downstream from specific extracellular signals. Here, we partially purified a Ras-inter acting protein with molecular mass of about 180 kDa (p180)from bovine brain membrane extract by glutathione S-transferase (GST)Ha-Ras affinity column chromatography. This protein bound to the GTP gamma S (guanosine 5'-(3-O-thio)triphosphate, a nonhydrolyzable GTP analog). GST-Ha-Ras affinity column but not to those containing GDP . GST-Ha-Ras or GTP gamma S . GST-Ha-Ras with a mutation in the effector domain (Ha-Ras(A38)). The amino acid sequences of the peptides derived from p180 were almost identical to those of human AF-6 that is identified as the fusion partner of the ALL-1 protein. The ALL-1/AF-6 chimeric protein is the critical product of the t (6:11) abnormality associated with some human leukemia. AF-6 has a GLGF/Dlg homology repeat (DHR)motif and shows a high degree of sequence similarity with Drosophila Canoe, which is assumed to function downstream from Notch in a common developmental pathway. The recombinant N-terminal domain of AF-6 and Canoe specifically interacted with GTP gamma S . GST-Ha-Ras. The known Ras target c-Raf-1 inhibited the interaction of AF 6 with GTP gamma S . GST-Ha-Ras. These results indicate that AF-6 and Canoe are putative targets for Ras.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/21 alle ore 18:23:36