Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
MUTATIONS OF THE P16 GENE IN GLIOMAS
Autore:
KYRITSIS AP; ZHANG BH; ZHANG W; XIAO M; TAKESHIMA H; BONDY ML; CUNNINGHAM JE; LEVIN VA; BRUNER J;
Indirizzi:
UNIV TEXAS,MD ANDERSON CANCER CTR,DEPT NEUROONCOL,1515 HOLCOMBE BLVD,BOX 100 HOUSTON TX 77030 UNIV TEXAS,MD ANDERSON CANCER CTR,DEPT EPIDEMIOL HOUSTON TX 77030 UNIV TEXAS,MD ANDERSON CANCER CTR,DEPT PATHOL HOUSTON TX 77030
Titolo Testata:
Oncogene
fascicolo: 1, volume: 12, anno: 1996,
pagine: 63 - 67
SICI:
0950-9232(1996)12:1<63:MOTPGI>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
SSCP ANALYSIS; CELLS;
Keywords:
ASTROCYTOMA; BRAIN TUMOR; MTS1; GLIOBLASTOMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
A.P. Kyritsis et al., "MUTATIONS OF THE P16 GENE IN GLIOMAS", Oncogene, 12(1), 1996, pp. 63-67

Abstract

In the present study we investigated the frequency of p16 gene exon 2mutations in 35 malignant gliomas, using either direct sequencing of the PCR products or cloning into the pCRII vector and sequencing of the cloned PCR products. No mutations were detected during direct sequencing of the PCR products. However, after sequencing ofindividual clones, we found multiple mutations in 5 tumors involving codons 73(GCC to ACC, Ala to Thr), 76 (GCC to GTC, Ala to Va), 85(GCT to ACT, Ala to Thr), 98(CAC to TAC, His to Tyr), 102 (GCG to GTG, Ala to Val), 106 (GTGto ATG, Val to Met), 107 (CGC to TGC, Arg to Cys), 127 (GCA to GTA, Ala to Val), 128 (CGG to TGG, Arg to Trp) and 136 (GGC to GAG, Gly to Asp). Mutations were found only in glioblastomas and were either C to Tor G to A transitions. Each mutation was detected in a small percentage of tumor cells (1.3-22%) using individual colony sequencing and southern hybridization with mutant oligonucleotides, consistent with the heterogenous cell population of glioblastomas. The presence of p16 gene mutations only in glioblastomas suggests that they are late events in glioma development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 19:05:09