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Titolo:
HUMAN MONONUCLEAR-CELLS EXPRESS 12-LX - COORDINATED MESSENGER-RNA REGULATION WITH 5-LX AND FLAP GENES
Autore:
KAMINSKI WE; JENDRASCHAK E; BAUMANN K; KIEFL R; FISCHER S; MARCUS AJ; BROEKMAN MJ; VONSCHACKY C;
Indirizzi:
UNIV WASHINGTON,DEPT PATHOL,BOX 357470,HSB J503 SEATTLE WA 98195 UNIV MUNICH,KLINIKUM INNENSTADT,MED KLIN W-8000 MUNICH GERMANY DEPT VET AFFAIRS MED CTR,DEPT MED NEW YORK NY 00000 DEPT VET AFFAIRS MED CTR,DEPT PATHOL NEW YORK NY 00000 CORNELL UNIV NEW YORK NY 00000 UNIV MUNICH,KLINIKUM GROSSHADERN,MED KLIN 2 W-8000 MUNICH GERMANY
Titolo Testata:
Blood
fascicolo: 1, volume: 87, anno: 1996,
pagine: 331 - 340
SICI:
0006-4971(1996)87:1<331:HME1-C>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN 5-LIPOXYGENASE GENE; FACTOR MESSENGER-RNA; N-3 FATTY-ACIDS; ARACHIDONIC-ACID; MOLECULAR-CLONING; MAMMALIAN LIPOXYGENASES; LEUKOTRIENE SYNTHESIS; HUMAN-LEUKOCYTES; 15-LIPOXYGENASE; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
W.E. Kaminski et al., "HUMAN MONONUCLEAR-CELLS EXPRESS 12-LX - COORDINATED MESSENGER-RNA REGULATION WITH 5-LX AND FLAP GENES", Blood, 87(1), 1996, pp. 331-340

Abstract

Lipoxygenases (LXs) catalyze formation of leukotrienes and hydroxy-eicosatetraenoic acids (HETEs), proinflammatory, and spasmogenic autacoids that are critical for host defense systems. We studied the expression and regulation of LX genes (12-LX, 5-LX, and 15-LX) and the 5-lipoxygenase activating protein (FLAP) in human mononuclear cells (MNC) andgranulocytes using a quantitative reverse transcription polymerase chain reaction (RT-PCR) technique. We show that 12-LX mRNA is constitutively expressed in resting platelet-free MNC. 12-LX gene expression wasupregulated by activation with lipopolysaccharide (LPS). The formation of 12-HETE was inducible with ionophore in MNC, as assessed by high-performance liquid chromatography (HPLC) and gas chromatography, and increased after LPS pretreatment. In addition to 12-LX, resting MNC expressed the genes for 5-LX and FLAP constitutively. Quantitative time course analyses of 12-LX, 5-LX, and FLAP gene expression suggested coregulation of 12-LX and FLAP mRNAs, and reciprocal regulation of 5-LX and FLAP mRNAs. During cell stimulation with LPS 5-LX mRNA levels remained unchanged, whereas FLAP gene expression increased. No 15-LX mRNA expression or 15-HETE formation was detectable in unstimulated and activated MNC. In contrast to MNC, quantitative RT-PCR mRNA analysis showedintermittent intraindividual expression of the 5-LX and FLAP genes inresting granulocytes, mRNAs for 12-LX and 15-LX were not expressed. On stimulation of granulocytes ex vivo, mRNA expression of 5-LX and FLAP was upregulated. Stimulation by LPS differed from that by ionophore A23187. Neither LPS nor ionophore induced gene expression of 12-LX or 15-LX in granulocytes. Our data indicate that resting human MNC and granulocytes express LX and FLAP genes in a cell-specific manner. Cell activation induces coordinated upregulation of 12-LX and FLAP genes in MNC, and 5-LX and FLAP genes in granulocytes, respectively. The constitutive expression of 12-LX mRNA, its upregulation on cell activation, and the formation of 12-HETE clearly indicate the presence of a functional 12-LX in human MNC. (C) 1996 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 17:38:41