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Titolo:
AVIAN-SARCOMA LEUKEMIA-VIRUS PROTEASE LINKED TO THE ADJACENT GAG POLYPROTEIN IS ENZYMATICALLY ACTIVE
Autore:
ARAD G; BARMEIR R; ALMOG N; CHOREV M; KOTLER M;
Indirizzi:
HEBREW UNIV JERUSALEM,HADASSAH MED SCH,DEPT MOLEC GENET IL-91010 JERUSALEM ISRAEL HEBREW UNIV JERUSALEM,HADASSAH MED SCH,DEPT MOLEC GENET IL-91010 JERUSALEM ISRAEL HEBREW UNIV JERUSALEM,HADASSAH MED SCH,DEPT PHARMACEUT CHEM IL-91010 JERUSALEM ISRAEL
Titolo Testata:
Virology
fascicolo: 2, volume: 214, anno: 1995,
pagine: 439 - 444
SICI:
0042-6822(1995)214:2<439:ALPLTT>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
RETROVIRAL PROTEASE; ESCHERICHIA-COLI; REVERSE-TRANSCRIPTASE; GENE; DNA; ACTIVATION; EXPRESSION; PROTEINASE; SEQUENCES; FUSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
G. Arad et al., "AVIAN-SARCOMA LEUKEMIA-VIRUS PROTEASE LINKED TO THE ADJACENT GAG POLYPROTEIN IS ENZYMATICALLY ACTIVE", Virology, 214(2), 1995, pp. 439-444

Abstract

The activity of avian sarcoma leukemia virus (ASLV) protease (PR) prior to its release from the precursor protein was determined by introducing mutations at the cleavage site between PR and the adjacent upstream nucleocapsid (No) protein. Gag DNA fragments containing these mutations were cloned into expression vectors and introduced into Escherichia coil in which the ASLV proteins were expressed. The dipeptide NC-PRcontaining these mutations did not undergo autoprocessing when expressed in bacterial cells and the fused proteins were devoid of enzymaticactivity. However, when the whole Gag polyprotein containing these mutations was expressed in bacterial cells, other PR cleavage sites in the viral Gag polyprotein underwent normal cleavage, indicating that the release of free PR is not a prerequisite for correct processing of the ASLV Gag precursor. (C) 1995 Academic Press, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:57:09