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Titolo:
A DUAL ROLE FOR BOTH CD40-LIGAND AND TNF-ALPHA IN CONTROLLING HUMAN B-CELL DEATH
Autore:
LENS SMA; TESSELAAR K; DENDRIJVER BFA; VANOERS MHJ; VANLIER RAW;
Indirizzi:
NETHERLANDS RED CROSS,BLOOD TRANSFUS SERV,CENT LAB,DEPT CLIN VIROIMMUNOL,PLESMANLAAN 125 1066 CX AMSTERDAM NETHERLANDS NETHERLANDS RED CROSS,BLOOD TRANSFUS SERV,CENT LAB,DEPT CLIN VIROIMMUNOL 1066 CX AMSTERDAM NETHERLANDS UNIV AMSTERDAM,EXPTL & CLIN IMMUNOL LAB AMSTERDAM NETHERLANDS UNIV AMSTERDAM,ACAD MED CTR,DEPT HEMATOL 1105 AZ AMSTERDAM NETHERLANDS
Titolo Testata:
The Journal of immunology
fascicolo: 2, volume: 156, anno: 1996,
pagine: 507 - 514
SICI:
0022-1767(1996)156:2<507:ADRFBC>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; GROWTH-FACTOR RECEPTOR; X-LINKED IMMUNODEFICIENCY; HUMAN LYMPHOCYTES-B; HUMAN T-CELLS; MONOCLONAL-ANTIBODY; SURFACE ANTIGEN; HYPER-IGM; MOLECULAR-CLONING; CROSS-LINKING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
64
Recensione:
Indirizzi per estratti:
Citazione:
S.M.A. Lens et al., "A DUAL ROLE FOR BOTH CD40-LIGAND AND TNF-ALPHA IN CONTROLLING HUMAN B-CELL DEATH", The Journal of immunology, 156(2), 1996, pp. 507-514

Abstract

Members of the TNF-R family are instrumental in controlling lymphoid cell death and survival, A major role in the regulation of murine and human B cell survival and differentiation has been attributed to CD40/CD40-ligand (CD40-L) interactions, but recent in vitro and in vivo data implicate that other receptor-ligand pairs might also be involved, We have used the human Burkitt lymphoma cell line Ramos as a model system to identify additional TNF-R/TNF family members that are implicatedin the regulation of B cell apoptosis, Ligation of B cell receptor (BCR) with anti-lgM mAb for 48 h induced apoptosis in approximately 68% of the Ramos B cells. Interestingly, not only CD40 mAb but also rTNF-alpha could efficiently inhibit BCR-induced B cell death, In addition, activated T cells also prevented BCR-triggered apoptosis, and this effect was inhibited completely by a combination of blocking Abs against CD40-L and TNF-alpha. In contrast to the strong effect of BCR ligation, APO-1 mAb induced apoptosis in only +/-18% of the Ramos cells after 48 h. Noticeably, addition of CD40 mAb or rTNF-alpha increased the percentage of cells (+/-46%) undergoing apoptosis, which correlated with an increase of Fas/APO-1 membrane expression induced by CD40 or TNF-R ligation. Taken together, we show that CD40/CD40-L and TNF-R/TNF-alphainteractions not only postpone or prevent B cell death, but are also involved in sensitizing B cells for Fas-ligand (Fas-L)-dependent death.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 09:33:24