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Titolo:
FAS-INDUCED AND ACTIVATION-INDUCED APOPTOSIS ARE REDUCED IN HUMAN T-CELLS PREACTIVATED IN THE PRESENCE OF TGF-BETA
Autore:
CERWENKA A; KOVAR H; MAJDIC O; HOLTER W;
Indirizzi:
ST ANNA CHILDRENS HOSP,CHILDRENS CANC RES INST,KINDERSPITALGASSE 6 A-1090 VIENNA AUSTRIA ST ANNA CHILDRENS HOSP,CHILDRENS CANC RES INST A-1090 VIENNA AUSTRIA UNIV VIENNA,INST IMMUNOL A-1010 VIENNA AUSTRIA
Titolo Testata:
The Journal of immunology
fascicolo: 2, volume: 156, anno: 1996,
pagine: 459 - 464
SICI:
0022-1767(1996)156:2<459:FAAAAR>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSFORMING GROWTH-FACTOR; TUMOR NECROSIS FACTOR; HUMAN LYMPHOCYTES-T; DNA FRAGMENTATION; PERIPHERAL-BLOOD; HELPER-CELLS; ANTIGEN; DEATH; MEMORY; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
A. Cerwenka et al., "FAS-INDUCED AND ACTIVATION-INDUCED APOPTOSIS ARE REDUCED IN HUMAN T-CELLS PREACTIVATED IN THE PRESENCE OF TGF-BETA", The Journal of immunology, 156(2), 1996, pp. 459-464

Abstract

The elimination of activated but not resting T cells involves apoptosis induced either by restimulation via the TCR/CD3 complex, CD2, or bysignaling through the Fas Ag. The factors regulating the shift of an apoptosis-resistant to an apoptosis-sensitive phenotype and vice versahave not so far been clarified, Here we report that TGF-beta(1), whenpresent during a PHA activation course, significantly increases viability of human T cells upon reculture in medium alone, following restimulation via CD2, CD3, or after triggering the Fas Ag, Using DNA gel electrophoresis and an in situ nick translation technique we further show that activation-induced and Fas-mediated apoptosis are reduced in T cells that were prestimulated with PHA plus TGF-beta(1), compared withcontrol cells prestimulated with PHA alone, Moreover, when PHA-preactivated T cells are further expanded in IL-2, inclusion of TGF-beta(1) results in higher cell yields at any timepoint from day 30 to 75 of cell culture compared with control cultures without TGF-beta(1). However, no differences in Fas or bcl-2 protein expression are found between cells stimulated in the absence or presence of TGF-beta(1). Together, our data identify TGF-beta(1), when present during an activation course, as an important viability factor possibly of importance for the generation of effector and/or long-lived memory T cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 05:10:53