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Titolo:
DEVELOPMENTAL PATTERN OF EXPRESSION AND GENOMIC ORGANIZATION OF THE CALPONIN-H1 GENE - A CONTRACTILE SMOOTH-MUSCLE CELL MARKER
Autore:
SAMAHA FF; IP HS; MORRISEY EE; SELTZER J; TANG ZH; SOLWAY J; PARMACEK MS;
Indirizzi:
UNIV CHICAGO,DEPT MED,MC 6088,5841 S MARYLAND AVE CHICAGO IL 60637 UNIV CHICAGO,DEPT MED CHICAGO IL 60637
Titolo Testata:
The Journal of biological chemistry
fascicolo: 1, volume: 271, anno: 1996,
pagine: 395 - 403
SICI:
0021-9258(1996)271:1<395:DPOEAG>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
TROPONIN-C GENE; ALPHA-ACTIN PROMOTER; CALMODULIN-BINDING; CHICKEN GIZZARD; BLOOD-VESSELS; F-ACTIN; PROTEIN; IDENTIFICATION; CALDESMON; AORTA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
75
Recensione:
Indirizzi per estratti:
Citazione:
F.F. Samaha et al., "DEVELOPMENTAL PATTERN OF EXPRESSION AND GENOMIC ORGANIZATION OF THE CALPONIN-H1 GENE - A CONTRACTILE SMOOTH-MUSCLE CELL MARKER", The Journal of biological chemistry, 271(1), 1996, pp. 395-403

Abstract

Calponin-h1 is a 34-kDa myofibrillar thin filament, actin-binding protein that is expressed exclusively in smooth muscle cells (SMCs) in adult animals, To examine the molecular mechanisms that regulate SMC specific gene expression, we have examined the temporal, spatial, and cell cycle-regulated patterns of expression of calponin-h1 gene expression and isolated and structurally characterized the murine calponin-h1 gene. Calponin-h1 mRNA is expressed exclusively in SMC-containing tissues in adult animals, During murine embryonic development, calponin-h1 gene expression is (i) detectable in E9.5 embryos in the dorsal aorta,cardiac outflow tract, and tubular heart, (ii) sequentially up-regulated in SMC containing tissues, and (iii) downregulated to non-detectable levels in the heart during late fetal development, In addition, thegene is expressed in resting rat aortic SMCs, but its expression is rapidly down-regulated when growth-arrested cells reenter phase G(1) ofthe cell cycle and proliferate, Calponin-h1 is encoded by a 10.7-kilobase single copy gene composed of seven exons, which is part of a multigene family, Transient transfection analyses demonstrated that 1.5 kilobases of calponin-h1 5'-flanking sequence is sufficient to program high level transcription of a luciferase reporter gene in cultured primary rat aortic SMCs and the smooth muscle cell line, A7r5. Taken together, these data suggest that the calponin-h1 gene will serve as an excellent model system with which to examine the molecular mechanisms that regulate SMC lineage specification, differentiation, and phenotypic modulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 06:31:34