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Titolo:
A CLATHRIN-BINDING SITE IN THE HINGE OF THE BETA-2 CHAIN OF MAMMALIANAP-2 COMPLEXES
Autore:
SHIH W; GALLUSSER A; KIRCHHAUSEN T;
Indirizzi:
HARVARD UNIV,SCH MED,DEPT CELL BIOL,200 LONGWOOD AVE BOSTON MA 02115 HARVARD UNIV,SCH MED,DEPT CELL BIOL BOSTON MA 02115 CTR BLOOD RES BOSTON MA 02115
Titolo Testata:
The Journal of biological chemistry
fascicolo: 52, volume: 270, anno: 1995,
pagine: 31083 - 31090
SICI:
0021-9258(1995)270:52<31083:ACSITH>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
VESICLE ASSEMBLY POLYPEPTIDES; FACTOR-II RECEPTOR; COATED VESICLES; PLASMA-MEMBRANE; PROTEIN COMPLEX; INVITRO BINDING; GOLGI MEMBRANES; ADAPTERS; ADAPTINS; DOMAINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
48
Recensione:
Indirizzi per estratti:
Citazione:
W. Shih et al., "A CLATHRIN-BINDING SITE IN THE HINGE OF THE BETA-2 CHAIN OF MAMMALIANAP-2 COMPLEXES", The Journal of biological chemistry, 270(52), 1995, pp. 31083-31090

Abstract

The assembly of cytosolic clathrin into the cytoplasmic face of coated pits and coated vesicles appears to be driven by the clathrin-associated protein (AP) complexes. We have previously shown that one of the large chains of the AP complexes, the beta chain, is sufficient to drive coat assembly in vitro. This chain consists of two domains, the amino-terminal trunk and the carboxyl-terminal ear, linked by a ''hinge. '' We report here that presence of the hinge in recombinant beta trunk or in recombinant beta ear fragments is essential for driving in vitroassembly of clathrin into coats. We have also used a binding assay tomap the clathrin-binding site by nested deletion of hinge sequences to a 50-residue region in the center of the hinge. This sequence is conserved in all known beta sequences from multicellular organisms. The interaction of a single beta hinge with a clathrin triskelion is weak, and we propose that recruitment of cytosolic clathrin to a forming coated pit involves simultaneous contacts between the legs of single clathrin trimers and the beta hinges of two or three membrane-bound AP complexes. Uncoating is likely to require interruption of these contacts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 10:31:04